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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Analytical development of a binuclear oxo-manganese complex bio-inspired on oxidase enzyme for doping control analysis of acetazolamide

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Author(s):
Machini, Wesley B. S. [1] ; Teixeira, Marcos F. S. [1]
Total Authors: 2
Affiliation:
[1] Sao Paulo State Univ UNESP, Fac Sci & Technol, Dept Chem & Biochem, Roberto Simonsen St 305, BR-19060900 Presidente Prudente, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: BIOSENSORS & BIOELECTRONICS; v. 79, p. 442-448, MAY 15 2016.
Web of Science Citations: 7
Abstract

A bio-inspired electrochemical sensor using a binuclear oxo-manganese complex was evaluated and applied in the detection of a substance associated with doping in sports: acetazolamide (ACTZ). Investigation was made of the influence of different experimental variables on the electrocatalytic oxidation of ACTZ by the bio-inspired sensor, such as pH and interfering species. The bio-inspired sensor showed the best response in the range from 5.00 x 10(-9) to 7.00 x 10(-8) mol L-1 ACTZ, with a linear range from 5.00 x 10(-9) to 2.50 x 10(-8) mol L-1 and a detection limit of 4.76 x 10(-9) mol L-1. The sensor exhibited characteristics similar to the Michaelis-Menten model of an enzymatic electrode, due to the use of a multinucleated complex of manganese with mu-oxo units, which was able to mimic the properties of enzymes with manganese as a cofactor in their composition, such as Mn-containing oxidase. The determination of ACTZ with the bio-inspired sensor was evaluated using three different synthetic biological fluids (plasma, saliva, and urine), demonstrating its viability for use with real samples. The analysis of ACTZ in real urine samples using the bio-inspired sensor, simulating the method adopted by the World Anti-Doping Agency, which revealed viable, suggesting a new and promising platform to be used in these analysis. (C) 2015 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 13/10469-6 - Electroanalytical application of the biomimetic manganese(IV) complex containing 2,3,5,6-tetrakis(2-pyridyl)pyrazine ligant
Grantee:Wesley Bruno da Silva Machini
Support Opportunities: Scholarships in Brazil - Master