Molecular and morphologic characterization of Sarcocystis felis (Apicomplexa: Sarcocystidae) in South American wild felids from Brazil

Autor(es)

Alberto Canon-Franco, William ^{[1, 2]} ; Lopez-Orozco, Natalia ^{[1, 3]} ; Christoff, Alexandre Uarth ^[4] ; de Castilho, Camila Schlieper ^[5] ; Pacheco de Araujo, Flavio Antonio ^[6] ; Verma, Shiv Kumar ^[7] ; Dubey, J. P. ^[7] ; Soares, Rodrigo Martins ^[1] ; Gennari, Solange Maria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Med Vet Prevent & Saude Anim, Fac Med Vet & Zootecn, Av Prof Dr Orlando Marques Paiva 87, Cidade Univ, BR-05508270 Sao Paulo, SP - Brazil

^[2] Univ Caldas, Fac Ciencias Agropecuarias, Dept Salud Anim, Calle 65 26-10, Manizales - Colombia

^[3] Univ Tecnol Pereira, Fac Ciencias Salud, Dept Ciencias Basicas, Grpo Infecc & Inmunidad, AA 097, La Julita, Pereira - Colombia

^[4] Univ Luterana Brasil, Dept Biol, Museu Ciencias Nat, Av Farroupilha 8001, BR-92425900 Canoas, RS - Brazil

^[5] Univ Sao Paulo, Inst Biociencias, Dept Ecol, Rua Mateo 321 Tray 14, BR-05508090 Sao Paulo, SP - Brazil

^[6] Univ Fed Rio Grande do Sul, Fac Vet, Dept Patol & Clin Vet, Av Bento Goncalves 9090, BR-91540000 Porto Alegre, RS - Brazil

^[7] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Bldg 1001, Beltsville, MD 20705 - USA

• Tipo de documento: Artigo Científico
• Fonte: Veterinary Parasitology; v. 217, p. 15-20, FEB 15 2016.
• Citações Web of Science: 0

Resumo

Wild felids are thought to share parasites with domestic cats. However, little is known of the coccidian parasites of wild felids. We investigated the presence of Sarcocystis spp. in tissues of 6 species of 90 Neotropical small felids killed in road accidents in the state of Rio Grande do Sul, Brazil by using microscopic and molecular techniques. Formalin-fixed tissues from 28 fends were examined, and Sarcocystis felis-like sarcocysts were detected in 4 wild cats (2 Puma yagouaroundi and 2 Leopardus guttulus). By transmission electron microscopy, sarcocysts from a P. yagouaroundi were identical to S. felis from domestic cats in the USA. Direct sequencing of PCR amplicons resulted the unambiguous sequences of the ITS-1 region from 18 of the 31 PCR positive wild cats; 5 sequences from each P. yagouaroundi, and Leopardus geoffroyi, 4 sequences from L. guttulus, and 2 sequences from each Leopardus wiedii, and Leopardus colocolo. Sequences analysis of ITS-1 region revealed the highest identiy (97-99%) with that of previously describe isolates of S. felis from domestic cats in the USA and identified them as S. felis. Tissues of 1 Leopardus pardalis tested by PCR and histology were negative. The phylogenetic relationship indicated that S. felis is quite different to species which employ opossums as their definitive host. This is the first report of S. felis infection in small wild felids from Brazil. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 12/25180-9 - Abordagem ecológica do ciclo silvestre de Toxoplama gondii em três biomas do Estado de Mato Grosso, Brasil. Avaliações moleculares da relação presa / predador.
• Beneficiário: William Alberto Canon Franco
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 10/52308-0 - Detecção molecular de coccídios da família Sarcocystidae (subfamílias Sarcocystinae e Toxoplasmatinae) em amostras teciduais de felinos neotropicais coletadas em museus do estado do Rio Grande do Sul
• Beneficiário: Solange Maria Gennari
• Modalidade de apoio: Auxílio à Pesquisa - Regular