Differential modulation of macrophage response elicited by glycoinositolphospholipids and lipophosphoglycan from Leishmania (Viannia) shawi

Autor(es)

Passero, Luiz F. D. ^[1] ; Assis, Rafael R. ^[2] ; da Silva, Thays N. F. ^[1] ; Nogueira, Paula M. ^[2] ; Macedo, Diego H. ^[2] ; Pessoa, Natalia L. ^[2] ; Campos, Marco A. ^[2] ; Laurenti, Marcia D. ^[1] ; Soares, Rodrigo P. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, BR-01246903 Sao Paulo, SP - Brazil

^[2] Fundacao Oswaldo Cruz, Ctr Pesquisas Rene Rachou, BR-30190002 Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Parasitology International; v. 64, n. 4, p. 32-35, AUG 2015.
• Citações Web of Science: 11

Resumo

In this work, some aspects of the glycobiology of Leishmattia shawl were examined, as it is a causative agent of cutaneous leishmaniasis in the New World. Additionally, the interaction of L. shawl's main glycoconjugates {[}lipophosphoglycan (LPG) and glycoinositolphospholipids (GIPLs)] with macrophages was evaluated in vitro. L. shawl LPG was devoid of side-chains in its repeat units, whereas monosaccharide analysis showed that GIPLs were suggestive of mannose-rich (type I or hybrid). In order to evaluate the biological roles of those molecules, BALB/c resident peritoneal macrophages were incubated with these glycoconjugates for 24 h, and the levels of nitric oxide (NO), tumor necrosis factor (TNF)-alpha, interleukin (IL)-12p70 and IL-10, were determined. In general, the GIPLs exhibited a greater proinflammatory role than the LPGs did. However, for the first time, the GIPLs from this species were able to trigger the production of IL-10, an anti-inflammatory cytokine. In conclusion, L shawl glycoconjugates were able to interact with the innate immune compartment. These data reinforce the role of parasite glycoconjugates during parasite and host cell interactions. (C) 2015 Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 12/10409-0 - Estudo das propriedades imunogênicas e protetoras de vacinas de terceira geração contra a leishmaniose tegumentar americana
• Beneficiário: Luiz Felipe Domingues Passero
• Linha de fomento: Auxílio à Pesquisa - Regular