Alternative Method for the Mass Rearing of Lutzomyia (Lutzomyia) cruzi (Diptera: Psychodidae) in a Laboratory Setting

Autor(es)

Oliveira, E. F. ^[1] ; Fernandes, W. S. ^[2] ; Oshiro, E. T. ^[2] ; Oliveira, A. G. ^[2] ; Galati, E. A. B. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Publ Hlth, Postgrad Program Publ Hlth, BR-01246904 Sao Paulo, SP - Brazil

^[2] Fed Univ Mato Grosso Sul UFMS, Ctr Biol & Hlth Sci, Parasitol Lab, BR-79090900 Campo Grande, MS - Brazil

^[3] Univ Sao Paulo, Sch Publ Hlth, Dept Epidemiol, BR-01246904 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Medical Entomology; v. 52, n. 5, p. 925-931, SEP 2015.
• Citações Web of Science: 3

Resumo

The understanding of the transmission dynamics of Leishmania spp. Ross as well as the epidemiology and spread of leishmaniasis is related to parasite-vector-host interactions. These interactions can be studied using specimens of a sand fly population reared in the laboratory, exposing individuals to experimental infection for the investigation of vector competence and parameters of the vectorial capacity of the species. The present study sought to describe an alternative method for the implantation of a Lutzomyia (Lutzomyia) cruzi colony with wild specimens captured in the municipality of Corumba, Brazil. With Method 1, engorged females were individualized for oviposition. The eggs were transferred to an acrylic petri dish with a layer of plaster on the bottom, on which food was placed after hatching of the first larvae. With Method 2, females were kept in groups for oviposition in containers, in which soil and food were placed on their bottom for the larvae. In addition, the exposure time of the larvae to light was reduced in comparison with Method 1. With Method 2, a significantly greater number of specimens of Lu. cruzi was obtained. The ratio between the number of emerged adults and the females followed for oviposition was 0.42 with Method 1 and 2.75 with Method 2. The optimization of the rearing conditions for Lu. cruzi will enable the establishment of a colony providing a sufficient number of specimens to develop experimental infection by Leishmania as well as vectorial competence and some parameters of the vectorial capacity of this sand fly. (AU)

• Processo FAPESP: 11/23414-0 - Capacidade vetorial de Lutzomyia (Lutzomyia) cruzi e Lutzomyia (Lutzomyia) forattinii (Diptera: Psychodidae) para Leishmania (Leishmania) infantum chagasi
• Beneficiário: Everton Falcão de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado