Exenatide and Sitagliptin Decrease Interleukin 1 beta, Matrix Metalloproteinase 9, and Nitric Oxide Synthase 2 Gene Expression But Does Not Reduce Alveolar Bone Loss in Rats With Periodontitis

Autor(es)

Moraes, Renata M. ^[1] ; Lima, Gabriela M. G. ^[1] ; Oliveira, Felipe E. ^[1] ; Brito, Ana Carolina V. ^[1] ; Pereira, Rodrigo C. ^[1] ; Oliveira, Luciane D. ^[1] ; Barros, Patricia P. ^[1] ; Franco, Gilson C. N. ^[2] ; Anbinder, Ana Lia ^[1]

Afiliação do(s) autor(es)

^[1] State Univ Sao Paulo, UNESP Univ Estadual Paulista, Inst Sci & Technol Sao Jose dos Campos, Dept Biosci & Oral Diag, Sao Paulo - Brazil

^[2] Univ Estadual Ponta Grossa, Dept Gen Biol, Ponta Grossa, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Periodontology; v. 86, n. 11, p. 1287-1295, NOV 2015.
• Citações Web of Science: 6

Resumo

Background: New drugs for the treatment of diabetes, glucagon-like peptide-1 (GLP-1) receptor agonists and inhibitors of dipeptidyl peptidase-4 (DPP-4) have shown pleiotropic effects on bone metabolism and anti-inflammatory properties. The aim of this study is to evaluate the effects of exenatide (GLP-1 agonist) and sitagliptin (DPP-4 inhibitor) during periodontitis induction by ligature insertion in rats. Methods: Forty rats were divided into four groups: 1) animals with induced periodontitis that received exenatide (EG); 2) animals with induced periodontitis that received sitagliptin (SG); 3) animals with induced periodontitis and without drug treatment (LG); and 4) animals without induced periodontitis and without drug treatment (controls). The drugs were administered for 28 days. On the day the animals were sacrificed, blood was collected for analysis of glucose and DPP-4 levels. The gene expressions of prostaglandin-endoperoxide synthase 2, tissue inhibitor of metalloproteinase 1, Dpp4, nitric oxide synthase 2 (Nos2), interleukin 1b (Il1b), and matrix metalloproteinase 9 (Mmp9) in the gingiva; support and alveolar bone loss; connective tissue attachment; and the quantity of gingival collagen were evaluated. Results: Exenatide and sitagliptin treatments have led to a lower percentage of weight gain but did not influence glycemia. Sitagliptin reduced the serum concentration of DPP-4. Interestingly, although the gene expression profile has revealed a downregulation of Mmp9, Nos2, and Il1b in both EG and SG compared to LG, a significant protective effect was not observed on alveolar bone and collagen tissue in this model. Conclusion: Regardless of the reduction of the expression of Il1b, Nos2, and Mmp9, the drugs were not effective in the stabilization or reduction of alveolar bone loss and collagen degradation in rats. (AU)

• Processo FAPESP: 13/17747-1 - Efeitos da exenatida e da sitagliptina na periodontite induzida em ratos
• Beneficiário: Renata Mendonça Moraes
• Modalidade de apoio: Bolsas no Brasil - Mestrado