Novel adipocyte aminopeptidases are selectively upregulated by insulin in healthy and obese rats

Autor(es)

Alponti, Rafaela Fadoni ^{[1, 2]} ; Alves, Patricia Lucio ^[2] ; Silveira, Paulo Flavio ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Physiol, Sao Paulo - Brazil

^[2] Inst Butantan, Unit Translat Endocrine Physiol & Pharmacol, Pharmacol Lab, Ave Vital Brasil 1500, BR-05503900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Endocrinology; v. 228, n. 2, p. 97-104, FEB 2016.
• Citações Web of Science: 0

Resumo

The lack of a complete assembly of the sensitivity of subcellular aminopeptidase (AP) activities to insulin in different pathophysiological conditions has hampered the complete view of the adipocyte metabolic pathways and its implications in these conditions. Here we investigated the influence of insulin on basic AP (APB), neutral puromycin-sensitive AP (PSA), and neutral puromycin-insensitive AP (APM) in high and low density microsomal and plasma membrane fractions from adipocytes of healthy and obese rats. Catalytic activities of these enzymes were fluorometrically monitoring in these fractions with or without insulin stimulus. Canonical traffic such as insulin-regulated AP was not detected for these novel adipocyte APs in healthy and obese rats. However, insulin increased APM in low density microsomal and plasma membrane fractions from healthy rats, APB in high density microsomal fraction from obese rats and PSA in plasma membrane fraction from healthy rats. A new concept of intracellular compartment-dependent upregulation of AP enzyme activities by insulin emerges from these data. This relatively selective regulation has pathophysiological significance, since these enzymes are well known to act as catalysts and receptor of peptides directly related to energy metabolism. Overall, the regulation of each one of these enzyme activities reflects certain dysfunction in obese individuals. (AU)

• Processo FAPESP: 10/01385-5 - Aminopeptidases, prolil oligopeptidase e peptídeos representativos em adipócitos de ratos obesos e privados de alimento
• Beneficiário: Paulo Flávio Silveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular