In vitro evaluation of adherence of Candida albicans, Candida glabrata, and Streptococcus mutans to an acrylic resin modified by experimental coatings

Autor(es)

Izumida, Fernanda Emiko ^[1] ; Moffa, Eduardo Buozi ^[1] ; Vergani, Carlos Eduardo ^[1] ; Machado, Ana Lucia ^[1] ; Jorge, Janaina Habib ^[1] ; Giampaolo, Eunice Teresinha ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Dept Dent Mat & Prosthodont, Araraquara Dent Sch, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOFOULING; v. 30, n. 5, p. 525-533, MAY 28 2014.
• Citações Web of Science: 9

Resumo

This study evaluated the effect of experimental coatings, containing zwitterion or hydrophilic monomers, on the adherence of Candida albicans, Candida glabrata, and Streptococcus mutans to an acrylic resin. Acrylic samples (smooth or rough surfaces) were left untreated (control) or coated with one of the following experimental coatings: 3-hydroxypropylmethacrylate (HP) or sulfobetaine methacrylate (S), at concentrations of 25, 30, or 35%. Half of the specimens were coated with saliva. The adhesion test was performed by incubating specimens in C. albicans, C. glabrata, and S. mutans suspensions at 37 degrees C for 90 min. The number of adhered microorganisms was determined by metabolic activity (XTT) and by cell viability (CFU). All coated specimens exhibited lower absorbance and CFU values compared to control specimens. Saliva and roughness did not promote microorganism adherence. An XPS analysis confirmed the modification in the chemical composition of the coatings in the experimental samples. These experimental coatings significantly reduced the adherence of C. albicans, C. glabrata and S. mutans to acrylic resin. (AU)

• Processo FAPESP: 10/00545-9 - Efetividade de vernizes fotopolimerizáveis experimentais e da escovação na redução da adesão de biofilme multi espécies em uma resina para base de prótese.
• Beneficiário: Fernanda Emiko Izumida
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/01528-6 - Efetividade de vernizes fotopolimerizáveis experimentais e da escovação na redução da adesão e formação de biofilmes em uma resina para base de prótese
• Beneficiário: Eunice Teresinha Giampaolo
• Modalidade de apoio: Auxílio à Pesquisa - Regular