Molecular and antibiotic susceptibility characterization of Aerococcus viridans isolated from porcine urinary infection

Autor(es)

Moreno, Luisa Z. ^[1] ; Matajira, Carlos E. C. ^[1] ; Gomes, Vasco T. M. ^[1] ; Silva, Ana Paula S. ^[1] ; Mesquita, Renan E. ^[1] ; Christ, Ana Paula G. ^[2] ; Sato, Maria Ines Z. ^[2] ; Moreno, Andrea M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Preventiva & Saude Anim, Av Prof Dr Orlando Marques de Paiva 87, Cidade Uni, Sao Paulo, SP - Brazil

^[2] Companhia Ambiental Estado Sao Paulo CETESB, Av Prof Frederico Hermann Jr 345, BR-05459900 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Veterinary Microbiology; v. 184, p. 7-10, FEB 29 2016.
• Citações Web of Science: 2

Resumo

Aerococcus viridans has been reported as a human and animal pathogen causing urinary tract infection, arthritis, pneumonia, meningitis and endocarditis. Routinely, A. viridans is not surveyed in clinical diagnosis laboratories and commonly is misidentified as other bacteria. There is no concrete data on the prevalence and impact of the pathogen to both human and animal health. In the present study, we report the isolation and molecular and antibiotic susceptibility characterization of A. viridans strains from porcine urinary infections. A total of 22 isolates were identified as A. viridans by MALDI-TOF MS and confirmed by 16S rRNA gene sequencing. Isolates were genotyped by single enzyme amplified fragments length polymorphism (SE-AFLP) that resulted in 19 clusters of which 81.2% were composed by single isolates. The high genetic heterogeneity corroborates previous studies and appears to be a particularity of A. viridans. The minimal inhibitory concentration (MIC) values also presented variability especially for ceftiofur, fluoroquinolones and aminoglycosides. The high MICs of aminoglycosides, tetracyclines and macrolides seen among the A. viridans corroborate previous reports and the widespread veterinary usage of these antibiotics demand attention for the implication of A. viridans infection to both human and animal health. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 13/17136-2 - Caracterização de estirpes de Leptospira isoladas no Brasil através de tipificação por eletroforese em campo pulsado (PFGE) e análise comparativa de genomas.
• Beneficiário: Luisa Zanolli Moreno
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/16946-0 - Caracterização fenotípica e genotípica de cepas de Salmonella entérica isoladas de carne suína no município de São Paulo.
• Beneficiário: Vasco Túlio de Moura Gomes
• Modalidade de apoio: Bolsas no Brasil - Doutorado