Expression of Secreted Aspartyl Proteinases in an Experimental Model of Candida albicans-Associated Denture Stomatitis

Autor(es)

Tobouti, Priscila Lie ^[1] ; Casaroto, Ana Regina ^[1] ; Couto de Almeida, Ricardo Sergio ^[2] ; Ramos, Solange de Paula ^[3] ; Dionisio, Thiago Jose ^[4] ; Porto, Vinicius Carvalho ^[5] ; Santos, Carlos Ferreira ^[4] ; Lara, Vanessa Soares ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Stomatol Oral Pathol, 9-75 Vila Univ, BR-17012901 Bauru - Brazil

^[2] Univ Estadual Londrina, Dept Microbiol, Londrina - Brazil

^[3] Univ Estadual Londrina, Dept Histol, Londrina - Brazil

^[4] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru - Brazil

^[5] Univ Sao Paulo, Bauru Sch Dent, Dept Prosthodont, Bauru - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PROSTHODONTICS-IMPLANT ESTHETIC AND RECONSTRUCTIVE DENTISTRY; v. 25, n. 2, p. 127-134, FEB 2016.
• Citações Web of Science: 4

Resumo

PurposeCandida albicans is known to produce secreted aspartyl proteinases (SAPs) to aid adhesion, invasion, and host tissue destruction. SAPs may contribute to denture stomatitis (DS) pathogenesis. The aim of this study was to develop an in vivo experimental model for Candida-associated DS that allows the analysis of SAP2, SAP5, and SAP9 expression by C. albicans from biofilm induced on the denture surface. Materials and MethodsThirty-five male Wistar rats were divided into three groups: control, denture, and denture/Candida group. The last two groups remained with dentures for 2, 4, and 6 days, with or without induced biofilm. SAP expression was concomitant with leukocyte counts as well as clinical and histological changes shown by animal palate. ResultsThe signs observed at 4 days in the denture/Candida group were clinically closer to the Candida-associated DS, showing a significant increase of neutrophils and decrease of lymphocytes in peripheral blood, presence of inflammation signs on the palate similar to DS Newton type I, and fungal invasion in the epithelial layer. Accordingly, the denture/Candida group at 4 days presented the highest relative expression of all SAPs studied. ConclusionThe results showed a coincidence between SAP expression and clinical, microscopic, and blood data. Finally, the molecular findings were consistent with the virulence capacities of C. albicans from biofilm formed on the denture resin, which possibly allowed epithelial invasion by the fungus. (AU)

• Processo FAPESP: 08/03539-0 - Estomatite por dentadura na população senescente: avaliação da ativação e da produção de citocinas pró e antiinflamatórias pelos monócitos
• Beneficiário: Vanessa Soares Lara
• Modalidade de apoio: Auxílio à Pesquisa - Regular