Dose-responses of Stem Cells from Human Exfoliated Teeth to Infrared LED Irradiation

Autor(es)

Ana Paula Silveira Turrioni ^[1] ; Liege Aldrovandi Montoro ^[2] ; Fernanda Gonçalves Basso ^[3] ; Leopoldina de Fátima Dantas de Almeida ^[4] ; Carlos Alberto de Souza Costa ^[5] ; Josimeri Hebling ^[6]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista. Araraquara School of Dentistry. Department of Pediatric Dentistry and Orthodontics - Brasil

^[2] Univ Estadual Paulista. Araraquara School of Dentistry. Department of Pediatric Dentistry and Orthodontics - Brasil

^[3] Univ Estadual Paulista. Araraquara School of Dentistry. Department of Physiology and Pathology - Brasil

^[4] Univ Estadual Paulista. Araraquara School of Dentistry. Department of Restorative Dentistry - Brasil

^[5] Univ Estadual Paulista. Araraquara School of Dentistry. Department of Physiology and Pathology - Brasil

^[6] Univ Estadual Paulista. Araraquara School of Dentistry. Department of Pediatric Dentistry and Orthodontics - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Dental Journal; v. 26, n. 4, p. 409-415, 2015-08-00.

Resumo

<p>Despite several reports regarding tissue regeneration, including pulp repair induced by different light sources, only limited data have been reported concerning the effects of light-emitting diodes (LED) on stem cells from human exfoliated deciduous teeth (SHEDs). The aim of this study was to evaluate the effects of different energy densities of infrared LED on the cell viability, number of cells and mineralized tissue production by SHEDs. SHEDs were obtained from near-exfoliation primary teeth (n=3), seeded in plain DMEM (104 cells/cm2), and irradiated by a LED prototype (LEDTable 850 nm, 40 mW/cm2) delivering 0 (control), 2, 4, 8, 15 or 30 J/cm2 (n=9). Cell viability (MTT assay), cell proliferation (trypan blue assay), and mineralized nodule (MN) formation (alizarin red stain) were assessed 12 and 72 h post-irradiation. Data were subjected to Kruskal-Wallis and Mann-Whitney tests (α=0.05). Cells irradiated with 2 or 4 J/cm2 exhibited higher metabolism at 72 h, and all energy densities provided increase in cell proliferation after 12 h. Regarding MN formation, the best results were observed at 72 h after SHED irradiation with 8 and 15 J/cm2. It was concluded that the cell viability, cell number and MN formation by pulp cells are enhanced after exposure to infrared LED irradiation. Overall, the greatest SHED biostimulation was obtained with 4 and 8 J/cm2.</p> (AU)

• Processo FAPESP: 13/17758-3 - Efeito da técnica de isolamento, fonte do tecido e passagem celular no número e fenótipo de células-tronco originárias da polpa dentária
• Beneficiário: Ana Paula Silveira Turrioni
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 11/13895-0 - Efeito direto e transdentinário do LED infravermelho sobre células-tronco da polpa dentária humana in vitro e sobre o complexo dentino-pulpar in vivo
• Beneficiário: Ana Paula Silveira Turrioni
• Modalidade de apoio: Bolsas no Brasil - Doutorado