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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Dose-responses of Stem Cells from Human Exfoliated Teeth to Infrared LED Irradiation

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Author(s):
Ana Paula Silveira Turrioni [1] ; Liege Aldrovandi Montoro [2] ; Fernanda Gonçalves Basso [3] ; Leopoldina de Fátima Dantas de Almeida [4] ; Carlos Alberto de Souza Costa [5] ; Josimeri Hebling [6]
Total Authors: 6
Affiliation:
[1] Univ Estadual Paulista. Araraquara School of Dentistry. Department of Pediatric Dentistry and Orthodontics - Brasil
[2] Univ Estadual Paulista. Araraquara School of Dentistry. Department of Pediatric Dentistry and Orthodontics - Brasil
[3] Univ Estadual Paulista. Araraquara School of Dentistry. Department of Physiology and Pathology - Brasil
[4] Univ Estadual Paulista. Araraquara School of Dentistry. Department of Restorative Dentistry - Brasil
[5] Univ Estadual Paulista. Araraquara School of Dentistry. Department of Physiology and Pathology - Brasil
[6] Univ Estadual Paulista. Araraquara School of Dentistry. Department of Pediatric Dentistry and Orthodontics - Brasil
Total Affiliations: 6
Document type: Journal article
Source: Brazilian Dental Journal; v. 26, n. 4, p. 409-415, 2015-08-00.
Abstract

<p>Despite several reports regarding tissue regeneration, including pulp repair induced by different light sources, only limited data have been reported concerning the effects of light-emitting diodes (LED) on stem cells from human exfoliated deciduous teeth (SHEDs). The aim of this study was to evaluate the effects of different energy densities of infrared LED on the cell viability, number of cells and mineralized tissue production by SHEDs. SHEDs were obtained from near-exfoliation primary teeth (n=3), seeded in plain DMEM (104 cells/cm2), and irradiated by a LED prototype (LEDTable 850 nm, 40 mW/cm2) delivering 0 (control), 2, 4, 8, 15 or 30 J/cm2 (n=9). Cell viability (MTT assay), cell proliferation (trypan blue assay), and mineralized nodule (MN) formation (alizarin red stain) were assessed 12 and 72 h post-irradiation. Data were subjected to Kruskal-Wallis and Mann-Whitney tests (α=0.05). Cells irradiated with 2 or 4 J/cm2 exhibited higher metabolism at 72 h, and all energy densities provided increase in cell proliferation after 12 h. Regarding MN formation, the best results were observed at 72 h after SHED irradiation with 8 and 15 J/cm2. It was concluded that the cell viability, cell number and MN formation by pulp cells are enhanced after exposure to infrared LED irradiation. Overall, the greatest SHED biostimulation was obtained with 4 and 8 J/cm2.</p> (AU)

FAPESP's process: 13/17758-3 - Effect of the isolation technique, source of tissue and cell passage on the number and phenotype of stem cells originating from dental pulp
Grantee:Ana Paula Silveira Turrioni
Support Opportunities: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 11/13895-0 - Transdentinal and direct effect of infrared LED on human dental pulp cells and pulp-dentin complex
Grantee:Ana Paula Silveira Turrioni
Support Opportunities: Scholarships in Brazil - Doctorate