Exploring the immune signalling pathway-related genes of the cattle tick Rhipicephalus microplus: From molecular characterization to transcriptional profile upon microbial challenge

Autor(es)

Rosa, Rafael D. ^{[1, 2]} ; Capelli-Peixoto, Janaina ^[1] ; Mesquita, Rafael D. ^[3] ; Kalil, Sandra P. ^[1] ; Pohl, Paula C. ^[1] ; Braz, Gloria R. ^[3] ; Fogaca, Andrea C. ^[1] ; Daffre, Sirlei ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, BR-05508900 Sao Paulo - Brazil

^[2] Univ Fed Santa Catarina, Dept Cell Biol Embryol & Genet, Lab Immunol Appl Aquaculture, BR-88040900 Florianopolis, SC - Brazil

^[3] Univ Fed Rio de Janeiro, Inst Chem, Dept Biochem, BR-21941909 Rio De Janeiro, RJ - Brazil

• Tipo de documento: Artigo Científico
• Fonte: DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY; v. 59, p. 1-14, JUN 2016.
• Citações Web of Science: 14

Resumo

In dipteran insects, invading pathogens are selectively recognized by four major pathways, namely Toll, IMD, JNK, and JAK/STAT, and trigger the activation of several immune effectors. Although substantial advances have been made in understanding the immunity of model insects such as Drosophila melanogaster, knowledge on the activation of immune responses in other arthropods such as ticks remains limited. Herein, we have deepened our understanding of the intracellular signalling pathways likely to be involved in tick immunity by combining a large-scale in silico approach with high-throughput gene expression analysis. Data from in si/ico analysis revealed that although both the Toll and JAK/STAT signalling pathways are evolutionarily conserved across arthropods, ticks lack central components of the D. melanogaster IMD pathway. Moreover, we show that tick immune signalling-associated genes are constitutively transcribed in BME26 cells (a cell lineage derived from embryos of the cattle tick Rhipicephalus microplus) and exhibit different transcriptional patterns in response to microbial challenge. Interestingly, Anaplasma marginale, a pathogen that is naturally transmitted by R. microplus, causes downregulation of immune-related genes, suggesting that this pathogen may manipulate the tick immune system, favouring its survival and vector colonization. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 10/50940-1 - Estudo de genes relacionados ao sistema imunológico do carrapato Rhipicephalus (Boophilus) microplus modulados pela riquétsia Anaplasma marginale
• Beneficiário: Thaís Duarte Bifano
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/23549-2 - Vias de sinalização celular envolvidas na resposta imunológica do carrapato bovino Rhipicephalus microplus
• Beneficiário: Janaína Capelli Peixoto
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/26450-2 - Caracterização molecular das interações entre carrapatos, riquétsias e hospedeiros vertebrados
• Beneficiário: Sirlei Daffre
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 11/22124-8 - Genômica funcional da interação entre Rhipicephalus (Boophilus) microplus e Anaplasma marginale
• Beneficiário: Thaís Duarte Bifano
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 13/25213-7 - Vias de sinalização celular envolvidas na resposta imunológica do carrapato bovino Rhipicephalus microplus
• Beneficiário: Janaína Capelli Peixoto
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado