Silkomics: Insight into the Silk Spinning Process of Spiders

Autor(es)

Aparecido dos Santos-Pinto, Jose Roberto ^{[1, 2]} ; Caviquioli Garcia, Ana Maria ^[2] ; Arcuri, Helen Andrade ^[2] ; Esteves, Franciele Grego ^[2] ; Salles, Heliana Clara ^[2] ; Lubec, Gert ^[1] ; Palma, Mario Sergio ^[2]

Afiliação do(s) autor(es)

^[1] Med Univ Vienna, Dept Pediat, A-1090 Vienna - Austria

^[2] Sao Paulo State Univ UNESP, Inst Biosci Rio Claro, Dept Biol, Ctr Study Social Insects, BR-13500 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PROTEOME RESEARCH; v. 15, n. 4, p. 1179-1193, APR 2016.
• Citações Web of Science: 12

Resumo

The proteins from the silk-producing glands were identified using both a bottom-up gel-based proteomic approach as well as from a shotgun proteomic approach. Additionally, the relationship between the functions of identified proteins and the spinning process was studied. A total of 125 proteins were identified in the major ampullate, 101 in the flagelliform, 77 in the aggregate, 75 in the tubuliform, 68 in the minor ampullate, and 23 in aciniform glands. On the basis of the functional classification using Gene Ontology, these proteins were organized into seven different groups according to their general function: (1) web silk proteins-spidroins, (ii) proteins related to the folding/conformation of spidroins, (iii) proteins that protect silk proteins from oxidative stress, (iv) proteins involved in fibrillar preservation of silks in the web, (v) proteins related to ion transport into and out of the glands during silk fiber spinning, (vi) proteins involved in prey captute and pre-digestion, and (vii) housekeeping proteins from all of the glands. Thus, a general mechanism of action for the identified proteins in the silk-producing glands from the Nephila clavipes spider was proposed; the current results also indicate that the webs play an active role in prey capture. (AU)

• Processo FAPESP: 10/19051-6 - Análise Estrutural das proteínas da seda da teia da aranha Nephila clavipes por abordagem proteômica.
• Beneficiário: José Roberto Aparecido dos Santos-Pinto
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/26451-9 - Bioprospecção e Análise Estrutural das Proteínas da Seda de Artrópodes por uma Abordagem Proteômica Utilizando um Sistema nanoLC-ESI-CID/ETD
• Beneficiário: José Roberto Aparecido dos Santos-Pinto
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/51684-1 - Biologia de sistemas como estratégia experimental para a descoberta de novos produtos naturais na fauna de artrópodes peçonhentos do Estado de São Paulo
• Beneficiário: Mario Sergio Palma
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático