Combination of Bioactive Polymeric Membranes and Stem Cells for Periodontal Regeneration: In Vitro and In Vivo Analyses

Autor(es):Mostrar menos -

Goncalves, Flavia ^[1] ; de Moraes, Miriam Santos ^[2] ; Ferreira, Lorraine Braga ^[3] ; Oliveira Carreira, Ana Claudia ^[4] ; Kossugue, Patricia Mayumi ^[4] ; Cidreira Boaro, Leticia Cristina ^[3] ; Bentini, Ricardo ^[1] ; da Silva Garcia, Celia Regina ^[2] ; Sogayar, Mari Cleide ^{[5, 4]} ; Arana-Chavez, Victor Elias ^[3] ; Catalani, Luiz Henrique ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, BR-05508000 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, BR-05508090 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Fac Odontol, Dept Biomat & Biol Oral, BR-05508000 Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Fac Med, NUCEL NETCEM Nucleo Terapia Celular & Mol, BR-05360130 Sao Paulo, SP - Brazil

^[5] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 11, n. 3 MAR 31 2016.
• Citações Web of Science: 7

Resumo

Regeneration of periodontal tissues requires a concerted effort to obtain consistent and predictable results in vivo. The aim of the present study was to test a new family of bioactive polymeric membranes in combination with stem cell therapy for periodontal regeneration. In particular, the novel polyester poly(isosorbide succinate-co-L-lactide) (PisPLLA) was compared with poly(L-lactide) (PLLA). Both polymers were combined with collagen (COL), hydroxyapatite (HA) and the growth factor bone morphogenetic protein-7 (BMP7), and their osteoinductive capacity was evaluated via in vitro and in vivo experiments. Membranes composed of PLLA/COL/HA or PisPLLA/COL/HA were able to promote periodontal regeneration and new bone formation in fenestration defects in rat jaws. According to quantitative real-time polymerase chain reaction (qRT-PCR) and Alizarin Red assays, better osteoconductive capacity and increased extracellular mineralization were observed for PLLA/COL/HA, whereas better osteoinductive properties were associated with PisPLLA/COL/HA. We concluded that membranes composed of either PisPLLA/COL/HA or PLLA/COL/HA present promising results in vitro as well as in vivo and that these materials could be potentially applied in periodontal regeneration. (AU)

• Processo FAPESP: 10/17698-2 - Uso de matrizes poliméricas eletrofiadas e células tronco de polpa dentária e de adipócitos na engenharia dos tecidos periodontais
• Beneficiário: Flavia Gonçalves
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/51295-5 - Genômica funcional em Plasmodium
• Beneficiário: Célia Regina da Silva Garcia
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 11/21442-6 - Polímeros sintéticos e naturais aplicados à engenharia de tecido
• Beneficiário: Luiz Henrique Catalani
• Modalidade de apoio: Auxílio à Pesquisa - Temático