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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Combination of Bioactive Polymeric Membranes and Stem Cells for Periodontal Regeneration: In Vitro and In Vivo Analyses

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Author(s):
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Goncalves, Flavia [1] ; de Moraes, Miriam Santos [2] ; Ferreira, Lorraine Braga [3] ; Oliveira Carreira, Ana Claudia [4] ; Kossugue, Patricia Mayumi [4] ; Cidreira Boaro, Leticia Cristina [3] ; Bentini, Ricardo [1] ; da Silva Garcia, Celia Regina [2] ; Sogayar, Mari Cleide [5, 4] ; Arana-Chavez, Victor Elias [3] ; Catalani, Luiz Henrique [1]
Total Authors: 11
Affiliation:
[1] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, BR-05508090 Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Fac Odontol, Dept Biomat & Biol Oral, BR-05508000 Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Fac Med, NUCEL NETCEM Nucleo Terapia Celular & Mol, BR-05360130 Sao Paulo, SP - Brazil
[5] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508000 Sao Paulo, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: PLoS One; v. 11, n. 3 MAR 31 2016.
Web of Science Citations: 7
Abstract

Regeneration of periodontal tissues requires a concerted effort to obtain consistent and predictable results in vivo. The aim of the present study was to test a new family of bioactive polymeric membranes in combination with stem cell therapy for periodontal regeneration. In particular, the novel polyester poly(isosorbide succinate-co-L-lactide) (PisPLLA) was compared with poly(L-lactide) (PLLA). Both polymers were combined with collagen (COL), hydroxyapatite (HA) and the growth factor bone morphogenetic protein-7 (BMP7), and their osteoinductive capacity was evaluated via in vitro and in vivo experiments. Membranes composed of PLLA/COL/HA or PisPLLA/COL/HA were able to promote periodontal regeneration and new bone formation in fenestration defects in rat jaws. According to quantitative real-time polymerase chain reaction (qRT-PCR) and Alizarin Red assays, better osteoconductive capacity and increased extracellular mineralization were observed for PLLA/COL/HA, whereas better osteoinductive properties were associated with PisPLLA/COL/HA. We concluded that membranes composed of either PisPLLA/COL/HA or PLLA/COL/HA present promising results in vitro as well as in vivo and that these materials could be potentially applied in periodontal regeneration. (AU)

FAPESP's process: 10/17698-2 - Use of electrospun polymer matrices and dental pulp and adipocytes stem cells in periodontal tissue engineering
Grantee:Flavia Gonçalves
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 11/51295-5 - Functional genomics in Plasmodium
Grantee:Célia Regina da Silva Garcia
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 11/21442-6 - Synthetic and natural polymers applied to tissue engineering
Grantee:Luiz Henrique Catalani
Support Opportunities: Research Projects - Thematic Grants