In vitro and ex vivo infection models help assess the molecular aspects of the interaction of Trichophyton rubrum with the host milieu

Autor(es)

de Aguiar Peres, Nalu Teixeira ^[1] ; da Silva, Larissa Gomes ^[1] ; Santos, Rodrigo da Silva ^[1] ; Jacob, Tiago Rinaldi ^[1] ; Persinoti, Gabriela Felix ^[1] ; Rocha, Lenaldo Branco ^[2] ; Falcao, Juliana Pfrimer ^[3] ; Rossi, Antonio ^[1] ; Martinez-Rossi, Nilce Maria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Triangulo Mineiro Fed Univ, Biol & Nat Sci Inst, BR-38025180 Uberaba, MG - Brazil

^[3] Univ Sao Paulo, Dept Clin Anal Toxicol & Food Sci, Ribeirao Preto Sch Pharmaceut Sci, BR-14049900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Medical Mycology; v. 54, n. 4, p. 420-427, MAY 2016.
• Citações Web of Science: 12

Resumo

Dermatophytes are fungal pathogens that cause cutaneous infections such as onychomycosis and athlete's foot in both healthy and immunocompromised patients. Trichophyton rubrum is the most prevalent dermatophyte causing human nail and skin infections worldwide, and because of its anthropophilic nature, animal infection models are limited. The purpose of this work was to compare the expression profile of T. rubrum genes encoding putative virulence factors during growth in ex vivo and in vitro infection models. The efficiency of the ex vivo skin infectionmodel was confirmed by scanning electron microscopy (SEM), which showed that the conidia had produced hyphae that penetrated into the epidermis. Quantitative RT-PCR (qRT-PCR) analysis showed that the expression of some genes is modulated in response to the infection model used, as compared to that observed in cells grown in glucose-containing media. We concluded that ex vivo infection models help assess the molecular aspects of the interaction of T. rubrum with the host milieu, and thus provide insights into the modulation of genes during infection. (AU)

• Processo FAPESP: 14/03847-7 - Caracterização molecular de mecanismos envolvidos na patogenicidade e sinalização celular em fungos
• Beneficiário: Nilce Maria Martinez-Rossi
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 09/08411-4 - Caracterização funcional de genes do fungo patogênico Trichophyton rubrum durante o processo de interação com células e moléculas do microambiente hospedeiro.
• Beneficiário: Nalu Teixeira de Aguiar Peres
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado