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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In vitro and ex vivo infection models help assess the molecular aspects of the interaction of Trichophyton rubrum with the host milieu

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Author(s):
de Aguiar Peres, Nalu Teixeira [1] ; da Silva, Larissa Gomes [1] ; Santos, Rodrigo da Silva [1] ; Jacob, Tiago Rinaldi [1] ; Persinoti, Gabriela Felix [1] ; Rocha, Lenaldo Branco [2] ; Falcao, Juliana Pfrimer [3] ; Rossi, Antonio [1] ; Martinez-Rossi, Nilce Maria [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Triangulo Mineiro Fed Univ, Biol & Nat Sci Inst, BR-38025180 Uberaba, MG - Brazil
[3] Univ Sao Paulo, Dept Clin Anal Toxicol & Food Sci, Ribeirao Preto Sch Pharmaceut Sci, BR-14049900 Ribeirao Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Medical Mycology; v. 54, n. 4, p. 420-427, MAY 2016.
Web of Science Citations: 12
Abstract

Dermatophytes are fungal pathogens that cause cutaneous infections such as onychomycosis and athlete's foot in both healthy and immunocompromised patients. Trichophyton rubrum is the most prevalent dermatophyte causing human nail and skin infections worldwide, and because of its anthropophilic nature, animal infection models are limited. The purpose of this work was to compare the expression profile of T. rubrum genes encoding putative virulence factors during growth in ex vivo and in vitro infection models. The efficiency of the ex vivo skin infectionmodel was confirmed by scanning electron microscopy (SEM), which showed that the conidia had produced hyphae that penetrated into the epidermis. Quantitative RT-PCR (qRT-PCR) analysis showed that the expression of some genes is modulated in response to the infection model used, as compared to that observed in cells grown in glucose-containing media. We concluded that ex vivo infection models help assess the molecular aspects of the interaction of T. rubrum with the host milieu, and thus provide insights into the modulation of genes during infection. (AU)

FAPESP's process: 14/03847-7 - Molecular characterization of mechanisms involved in pathogenicity and cell signaling in fungi
Grantee:Nilce Maria Martinez-Rossi
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 09/08411-4 - Functional characterization of genes from the pathogenic fungus Trichophyton rubrum during interaction with host microenvironment cells and molecules.
Grantee:Nalu Teixeira de Aguiar Peres
Support Opportunities: Scholarships in Brazil - Post-Doctoral