| Texto completo | |
| Autor(es): |
Arias, L. S.
[1]
;
Delbem, A. C. B.
[1]
;
Fernandes, R. A.
[2]
;
Barbosa, D. B.
[2]
;
Monteiro, D. R.
[1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Estadual Paulista UNESP, Aracatuba Dent Sch, Dept Pediat Dent & Publ Hlth, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil
[2] Univ Estadual Paulista UNESP, Aracatuba Dent Sch, Dept Dent Mat & Prosthodont, BR-16015050 Aracatuba, SP - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of Applied Microbiology; v. 120, n. 5, p. 1240-1249, MAY 2016. |
| Citações Web of Science: | 14 |
| Resumo | |
AimThis study aimed to evaluate the effect of tyrosol on the formation of single and mixed biofilms of Candida albicans ATCC 10231, Candida glabrata ATCC 90030 and Streptococcus mutans ATCC 25175 formed on acrylic resin (AR) and hydroxyapatite (HA) surfaces. Methods and ResultsSingle and mixed biofilms were formed on AR and HA in the presence of tyrosol at 50, 100 and 200mmoll(-1), during 48h. Next, antimicrobial activity was assessed through metabolic activity (XTT reduction assay) and the number of colony-forming units (CFUs). Scanning electron microscopy observations were performed in order to analyse biofilm structure. Tyrosol, mainly at 200mmoll(-1), significantly decreased the metabolic activity and number of CFUs for all single and mixed-species biofilms formed on both surfaces. SEM images suggested cell damage caused by tyrosol. ConclusionTyrosol showed inhibitory effects against biofilms formed by important oral pathogens. Significance and Impact of the StudyThis is the first study showing the antibiofilm effect of tyrosol on Candida species and Strep.mutans in single and mixed cultures. These results may be useful in the development of topical therapies focused on preventing biofilm-associated oral diseases, such as denture stomatitis and dental caries. (AU) | |
| Processo FAPESP: | 13/10285-2 - Efeito do tirosol sobre fatores de virulência de Candida albicans, Candida glabrata e Streptococcus mutans |
| Beneficiário: | Alberto Carlos Botazzo Delbem |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 14/05507-9 - Efeito do Tirosol na produção de ácido láctico e na capacidade de formação de biofilmes por Streptococcus mutans |
| Beneficiário: | Laís Salomão Arias |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 13/03273-8 - Efeito do Tirosol sobre fatores de virulência de Candida albicans, Candida glabrata e Streptococcus mutans |
| Beneficiário: | Douglas Roberto Monteiro |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |