Activity of tyrosol against single and mixed-species oral biofilms

Author(s)

Arias, L. S. ^[1] ; Delbem, A. C. B. ^[1] ; Fernandes, R. A. ^[2] ; Barbosa, D. B. ^[2] ; Monteiro, D. R. ^[1]

Affiliation

^[1] Univ Estadual Paulista UNESP, Aracatuba Dent Sch, Dept Pediat Dent & Publ Hlth, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[2] Univ Estadual Paulista UNESP, Aracatuba Dent Sch, Dept Dent Mat & Prosthodont, BR-16015050 Aracatuba, SP - Brazil

• Document type: Journal article
• Source: Journal of Applied Microbiology; v. 120, n. 5, p. 1240-1249, MAY 2016.
• Web of Science Citations: 14

Abstract

AimThis study aimed to evaluate the effect of tyrosol on the formation of single and mixed biofilms of Candida albicans ATCC 10231, Candida glabrata ATCC 90030 and Streptococcus mutans ATCC 25175 formed on acrylic resin (AR) and hydroxyapatite (HA) surfaces. Methods and ResultsSingle and mixed biofilms were formed on AR and HA in the presence of tyrosol at 50, 100 and 200mmoll(-1), during 48h. Next, antimicrobial activity was assessed through metabolic activity (XTT reduction assay) and the number of colony-forming units (CFUs). Scanning electron microscopy observations were performed in order to analyse biofilm structure. Tyrosol, mainly at 200mmoll(-1), significantly decreased the metabolic activity and number of CFUs for all single and mixed-species biofilms formed on both surfaces. SEM images suggested cell damage caused by tyrosol. ConclusionTyrosol showed inhibitory effects against biofilms formed by important oral pathogens. Significance and Impact of the StudyThis is the first study showing the antibiofilm effect of tyrosol on Candida species and Strep.mutans in single and mixed cultures. These results may be useful in the development of topical therapies focused on preventing biofilm-associated oral diseases, such as denture stomatitis and dental caries. (AU)

• FAPESP's process: 13/10285-2 - Effect of tyrosol on virulence factors of Candida albicans, Candida glabrata and Streptococcus mutans
• Grantee: Alberto Carlos Botazzo Delbem
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/05507-9 - Effect of Tyrosol on the lactic acid production and on the ability to form biofilms by Streptococcus mutans
• Grantee: Laís Salomão Arias
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 13/03273-8 - Effect of Tyrosol on virulence factors of Candida albicans, Candida glabrata and Streptococcus mutans
• Grantee: Douglas Roberto Monteiro
• Support Opportunities: Scholarships in Brazil - Post-Doctoral