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Synthesis, characterization and photodynamic effects of polymeric nanoparticle-encapsulated curcumin on in vitro biofilms of Candida albicans and on a murine model of oral candidosis


The aim of this study is to formulate and evaluate the photodynamic effect of curcumin (CUR) encapsulated in polymeric nanoparticles (NP) on in vitro biofilms of Candida albicans and on a murine model of oral candidosis. CUR-NP will be synthesized using poly-lactic acid (PLA), and dextran sulfate (DEX). To characterize this solution, the physicochemical properties (size, polydispersity and zeta potential) will be determined and tests of encapsulation efficiency, absorption spectrum, photostability (photodegradation of CUR) and photodynamic activity (photobleaching of singlet oxygen reagent and scavenger) will be also performed. Next, the effects of CUR-NP-mediated Photodynamic Therapy (PDT) against monospecies biofilms of C. albicans and polymicrobial biofilm of C. albicans associated with Streptococcus mutans and methicillin-resistant Staphylococcus aureus, cultured in vitro for 48 hours, will be assessed by quantification of colonies (CFU/mL), total biomass (crystal violet staining), scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). On CLSM, penetration of photosensitizer into the biofilm, cell viability, and structure of biofilm polymeric matrix will be analyzed. Each evaluation will be carried out in triplicate on three separate occasions and compared to free CUR. Additionally, the effectiveness of CUR-NP-mediated PDT will be evaluated in a murine model of oral candidiasis. Female mice (n = 35) will be immunosuppressed and inoculated with C. albicans to induce oral candidosis. After PDT, the fungal viability (CFU/mL) will be determined, the animals will be sacrificed and histological analysis of the tongue will be performed. Data will be statistically analyzed. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
TRIGO GUTIERREZ, JEFFERSSON KRISHAN; ZANATTA, GABRIELA CRISTINA; MIRA ORTEGA, ANA LAURA; CUBA BALASTEGUI, MARIA ISABELLA; SANITA, PAULA VOLPATO; PAVARINA, ANA CLAUDIA; BARBUGLI, PAULA ABOUD; DE OLIVEIRA MIMA, EWERTON GARCIA. Encapsulation of curcumin in polymeric nanoparticles for antimicrobial Photodynamic Therapy. PLoS One, v. 12, n. 11, . (14/18345-7, 13/23165-5, 13/07276-1)
SAKIMA, VINICIUS TATSUYUJI; BARBUGLI, PAULA ABOUD; CERRI, PAULO SERGIO; CHORILLI, MARLUS; CARMELLO, JULIANA CABRINI; PAVARINA, ANA CLAUDIA; DE OLIVEIRA MIMA, EWERTON GARCIA. Antimicrobial Photodynamic Therapy Mediated by Curcumin-Loaded Polymeric Nanoparticles in a Murine Model of Oral Candidiasis. Molecules, v. 23, n. 8, . (13/23165-5, 13/07276-1)

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Filed patent(s) as a result of this research project

PROCESSO DE OBTENÇÃO DE NANOPARTÍCULAS POLIMÉRICAS ANIÔNICAS E CATIÔNICAS DE CURCUMINA, NANOPARTÍCULAS OBTIDAS E SEUS USOS BR1020160156319 - Universidade Estadual Paulista (UNESP) . Solicitação em análise e dentro do prazo legal de sigilo previsto na legislação - July 2016, 04