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Photodynamic effects of polymeric nanoparticle-encapsulated curcumin on multispecies biofilm of methicillin-resistant Staphylococcus aureus and Candida albicans

Grant number: 14/18345-7
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): December 01, 2014
Effective date (End): April 30, 2016
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal Investigator:Ewerton Garcia de Oliveira Mima
Grantee:Ana Laura Mira Ortega
Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil


The aim of this study is to evaluate the photodynamic effect of curcumin (CUR) encapsulated in polymeric nanoparticles (NP) on multispecies biofilms of methicillin-resistant Staphylococcus aureus (MRSA) and Candida albicans. CUR-NP will be synthesized using poly-lactic acid (PLA), and dextran sulfate (DEX). To characterize this solution, the physicochemical properties (size, polydispersity and zeta potential) will be determined. Standard strains of MRSA (ATCC 33591) and C. albicans (ATCC 90028) will be cultivated for the development of multispecies biofilm for 48 hours at 37ºC. Next, the effects of Photodynamic Therapy (PDT) mediated by CUR-NP and blue LED light against biofilm will be assessed by quantification of colonies (CFU/mL), total biomass (crystal violet staining) and scanning electron microscopy (SEM). Each evaluation will be carried out in triplicate on three separate occasions and compared to free CUR. Data will be statistically analyzed.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
TRIGO GUTIERREZ, JEFFERSSON KRISHAN; ZANATTA, GABRIELA CRISTINA; MIRA ORTEGA, ANA LAURA; CUBA BALASTEGUI, MARIA ISABELLA; SANITA, PAULA VOLPATO; PAVARINA, ANA CLAUDIA; BARBUGLI, PAULA ABOUD; DE OLIVEIRA MIMA, EWERTON GARCIA. Encapsulation of curcumin in polymeric nanoparticles for antimicrobial Photodynamic Therapy. PLoS One, v. 12, n. 11, . (14/18345-7, 13/23165-5, 13/07276-1)

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