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Effect of tyrosol on the lactic acid production and on the ability to form biofilms by Streptococcus mutans

Grant number: 14/05507-9
Support type:Scholarships in Brazil - Master
Effective date (Start): June 01, 2014
Effective date (End): February 29, 2016
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Douglas Roberto Monteiro
Grantee:Laís Salomão Arias
Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil


The present study aims to evaluate the effect of different Tyrosol concentrations on the lactic acid production and on the ability to form biofilms by Streptococcus mutans. First, according to the microdilution method, it will determined the minimum inhibitory concentration of Tyrosol capable of inhibiting the growth of S. mutans in the planktonic state. After, S. mutans biofilms will be formed (for 48 hours) in the presence of different Tyrosol concentrations on specimens of hydroxyapatite within 24-wells microtiter plates. Subsequently, the lactic acid production by S. mutans will be measured by the Clinpro Cario L-Pop Kit. The effect of Tyrosol on the biofilm formation ability will be quantified by counting the number of culturable cells, total biomass (crystal violet staining assay) and metabolic activity of the cells. Moreover, the effect of Tyrosol on preformed biofilms will be assessed through the previously mentioned assays. Biofilm structure after treatment with Tyrosol will be visualized by scanning electron microscopy. Controls devoid Tyrosol (negative) and chlorhexidine digluconate at 0.025% (positive control) will be included in all analyzes, and all tests will be performed in triplicate on three different occasions. Data will be analyzed to employ the most appropriate statistical test. P < .05 will be considered statistically significant.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MONTEIRO, DOUGLAS ROBERTO; ARIAS, LAIS SALOMAO; FERNANDES, RENAN APARECIDO; STRAIOTO, FABIANA GOUVEIA; BARBOSA, DEBORA BARROS; PESSAN, JULIANO PELIM; BOTAZZO DELBEM, ALBERTO CARLOS. Role of tyrosol on Candida albicans, Candida glabrata and Streptococcus mutans biofilms developed on different surfaces. AMERICAN JOURNAL OF DENTISTRY, v. 30, n. 1, p. 35-39, FEB 2017. Web of Science Citations: 2.
ARIAS, L. S.; DELBEM, A. C. B.; FERNANDES, R. A.; BARBOSA, D. B.; MONTEIRO, D. R. Activity of tyrosol against single and mixed-species oral biofilms. Journal of Applied Microbiology, v. 120, n. 5, p. 1240-1249, MAY 2016. Web of Science Citations: 15.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ARIAS, Laís Salomão. Efeito do tirosol sobre biofilmes de streptococcus mutans. 2015. Master's Dissertation - Universidade Estadual Paulista "Júlio de Mesquita Filho" Faculdade de Odontologia (Campus de Araçatuba)..

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