Effect of farnesol on virulence factors of Candida albicans and Streptococcus mutans

• Grant number: 13/23592-0
• Support type: Scholarships in Brazil - Master
• Effective date (Start): June 01, 2014
• Effective date (End): November 30, 2015
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal researcher: Debora Barros Barbosa
• Grantee: Renan Aparecido Fernandes
• Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The present study aims to evaluate the effect of different farnesol concentrations on a hydrolytic enzyme (proteinase, phospholipase and hemolytic) production by Candida albicans, on the lactic acid production by Streptococcus mutans, and on the ability to form single and mixed biofilms of those species. First, according to the microdilution method, it will determined the minimum inhibitory concentration of Farnesol capable of inhibiting the growth of each strain in the planktonic state. After, single and mixed biofilms of C. albicans and S. mutans will be formed (for 48 hours) in the presence of different farnesol concentrations within 96-wells microtiter plates. Subsequently, the enzymatic activity of proteinase phospholipase and hemolytic of C. albicans will be evaluated by using specific culture medium for each type of hydrolytic enzyme, while the lactic acid production by S. mutans will be measured by the Clinpro Cario L-Pop Kit. The effect of Farnesol on the biofilm formation ability will be quantified by counting the number of Culturable Cells (CFU), total biomass (crystal violet staining assay) and metabolic activity of the cells (XTT). Biofilm structure under treatment with farnesol will be visualized by scanning electron microscopy. Controls devoid Farnesol will be included in all analyzes, and all tests will be performed in triplicate on three different occasions. Data will be analyzed to employ the most appropriate statistical test. P < .05 will be considered statistically significant. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERNANDES, RENAN APARECIDO; MONTEIRO, DOUGLAS ROBERTO; ARIAS, LAIS SALOMAO; FERNANDES, GABRIELA LOPES; BOTAZZO DELBEM, ALBERTO CARLOS; BARBOSA, DEBORA BARROS. Virulence Factors in Candida albicans and Streptococcus mutans Biofilms Mediated by Farnesol. INDIAN JOURNAL OF MICROBIOLOGY, v. 58, n. 2, p. 138-145, JUN 2018. Web of Science Citations: 4.

FERNANDES, RENAN APARECIDO; MONTEIRO, DOUGLAS ROBERTO; ARIAS, LAIS SALOMAO; FERNANDES, GABRIELA LOPES; BOTAZZO DELBEM, ALBERTO CARLOS; BARBOSA, DEBORA BARROS. Biofilm formation by Candida albicans and Streptococcus mutans in the presence of farnesol: a quantitative evaluation. BIOFOULING, v. 32, n. 3, p. 329-338, 2016. Web of Science Citations: 16.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FERNANDES, Renan Aparecido. Efeito do farnesol sobre fatores de virulência de Candida albicans e Streptococcus mutans. 2015. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba Araçatuba.