Co-cultivation of Aspergillus nidulans Recombinant Strains Produces an Enzymatic Cocktail as Alternative to Alkaline Sugarcane Bagasse Pretreatment

Autor(es)

Lima, Matheus S. ^[1] ; Damasio, Andre R. de L. ^[2] ; Crnkovic, Paula M. ^[3] ; Pinto, Marcelo R. ^[4] ; da Silva, Ana M. ^[5] ; da Silva, Jeanc. R. ^[6] ; Segato, Fernando ^[7] ; de Lucas, Rosymar C. ^{[8, 1]} ; Jorge, Joao A. ^[1] ; Polizeli, Maria de L. T. de M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Dept Biol, Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Mech Engn, Sao Paulo - Brazil

^[4] Univ Uberaba, Lab Biopathol & Mol Biol, Uberaba - Brazil

^[5] Univ Fed Campina Grande, Cuite - Brazil

^[6] Fed Inst Educ Sci & Technol Sao Paulo, Sao Paulo - Brazil

^[7] Univ Sao Paulo, Dept Biotechnol, Engn Sch Lorena, Sao Paulo - Brazil

^[8] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN MICROBIOLOGY; v. 7, APR 28 2016.
• Citações Web of Science: 5

Resumo

Plant materials represent a strategic energy source because they can give rise to sustainable biofuels through the fermentation of their carbohydrates. A clear example of a plant-derived biofuel resource is the sugar cane bagasse exhibiting 60-80% of fermentable sugars in its composition. However, the current methods of plant bioconversion employ severe and harmful chemical/physical pretreatments raising biofuel cost production and environmental degradation. Replacing these methods with co-cultivated enzymatic cocktails is an alternative. Here we propose a pretreatment for sugarcane bagasse using a multi-enzymatic cocktail from the co-cultivation of four Aspergillus nidulans recombinant strains. The co-cultivation resulted in the simultaneous production of GH51 arabinofuranosidase (AbfA), GH11 endo-1,4-xylanase (XInA), GH43 endo-1,5-arabinanase (AbnA) and GH12 xyloglucan specific endo-beta-1,4-glucanase (XegA). This core set of recombinant enzymes was more efficient than the alternative alkaline method in maintaining the cellulose integrity and exposing this cellulose to the following saccharification process. Thermogravimetric and differential thermal analysis revealed residual byproducts on the alkali pretreated biomass, which were not found in the enzymatic pretreatment. Therefore, the enzymatic pretreatment was residue-free and seemed to be more efficient than the applied alkaline method, which makes it suitable for bioethanol production. (AU)

• Processo FAPESP: 13/18910-3 - Secreção de glicoproteínas heterólogas em Aspergillus: efeito do padrão de glicosilação em parâmetros funcionais de glicosil hidrolases
• Beneficiário: André Ricardo de Lima Damasio
• Modalidade de apoio: Bolsas no Brasil - Programa BIOEN - Jovens Pesquisadores
• Processo FAPESP: 12/20549-4 - Secreção de glicoproteínas heterólogas em Aspergillus: efeito do padrão de glicosilação em parâmetros funcionais de glicosil hidrolases
• Beneficiário: André Ricardo de Lima Damasio
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Jovens Pesquisadores
• Processo FAPESP: 10/52322-3 - Bioprospecção de fungos filamentosos produtores de holoenzimas com aplicação em biorefinaria
• Beneficiário: Maria de Lourdes Teixeira de Moraes Polizeli
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular