Predicting delayed kidney graft function with gene expression in preimplantation biopsies and first-day posttransplant blood

Autor(es)

Mourao, Tuila B. ^{[1, 2]} ; Mine, Karina L. ^[2] ; Campos, Erika F. ^{[1, 2]} ; Medina-Pestana, Jose O. ^{[1, 3]} ; Tedesco-Silva, Helio ^[3] ; Gerbase-DeLima, Maria ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Sao Paulo, SP - Brazil

^[2] Inst Immunogenet AFIP, Rua Loefgreen 1235, BR-04040031 Sao Paulo, SP - Brazil

^[3] Hosp Rim & Hipertensao, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: HUMAN IMMUNOLOGY; v. 77, n. 4, p. 353-357, APR 2016.
• Citações Web of Science: 0

Resumo

The purpose of this study was to investigate possible markers for predicting delayed graft function (DGF). To this end we analyzed, in pre-implantation biopsies (PIB) and in first-day post-Tx peripheral blood mononuclear cells (PBMC), the expression of five genes (ACSL4, CUBN, DEFB1, FABP3, GK) through real-time TaqMan PCR assays. These genes were selected from a large scale gene expression study in PIB. DEFB1, FABP3 and GK expression levels in PIB were lower in cases with DGF and, in a multivariate analysis which included these genes and clinical variables, only FABP3 expression remained independently associated with DGF. FABP3 expression lower than-1.32 units of relative expression conferred an odds ratio for DGF of 41.1. Compared to the PBMC of recipients without DGF, recipients with prolonged DGF (pDGF) had lower ACSL4 and higher DEFB1 expression levels. In a multivariate analysis, including PBMC gene expression levels of ACSL4, DEFB1 and TLR4 (data from a previous study with the same patients) and clinical variables, only TLR4 remained independently associated with pDGF. In summary, this study revealed FABP3 expression in PIB as a marker for DGF and disclosed new genes possibly involved in the pathogenesis of DGF. (C) 2016 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 11/15279-5 - Análise de expressão gênica, em biópsia renal pré-implantação e em sangue periférico coletado no primeiro dia após o transplante, e a relação com a ocorrência do retardo da função do enxerto renal.
• Beneficiário: Tuíla Bittencourt Mourão
• Modalidade de apoio: Bolsas no Brasil - Mestrado