Interkingdom cooperation between Candida albicans, Streptococcus oralis and Actinomyces oris modulates early biofilm development on denture material

Autor(es)

Cavalcanti, Indira M. G. ^{[1, 2]} ; Nobbs, Angela H. ^[2] ; Ricomini-Filho, Antonio Pedro ^[3] ; Jenkinson, Howard F. ^[2] ; Del Bel Cury, Altair A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Prosthodont & Periodontol, Limeira Ave 901, BR-13414903 Piracicaba, SP - Brazil

^[2] Univ Bristol, Sch Oral & Dent Sci, Lower Maudlin St, Bristol BS1 2LY, Avon - England

^[3] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Ave Limeira 901, BR-13414903 Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PATHOGENS AND DISEASE; v. 74, n. 3 APR 2016.
• Citações Web of Science: 12

Resumo

Candida-associated stomatitis affects up to 60% of denture wearers, and Candida albicans remains the most commonly isolated fungal species. The oral bacteria Actinomyces oris and Streptococcus oralis are abundant in early dental plaque. The aims of this study were to determine the effects of S. oralis and A. oris on the development of C. albicans biofilms on denture material. Resin discs were coated with saliva and at early (1.5 h) or later (24 h) stages of biofilm development, cell numbers of each species were determined. Spatial distribution of microorganisms was visualized by confocal scanning laser microscopy of biofilms labelled by differential fluorescence or by fluorescence in situ hybridization. Interkingdom interactions underpinning biofilm development were also evaluated planktonically utilizing fluorescence microscopy. Synergistic interactions between all three species occurred within biofilms and planktonically. Bacterial cells coaggregated with each other and adhered singly or in coaggregates to C. albicans hyphal filaments. Streptococcus oralis appeared to enhance hyphal filament production and C. albicans biovolume was increased 2-fold. Concomitantly, cell numbers of S. oralis and A. oris were enhanced by C. albicans. Thus, cooperative physical and metabolic processes occurring between these three microbial species intensify pathogenic plaque communities on denture surfaces. (AU)

• Processo FAPESP: 13/15884-1 - Avaliação das interações microbianas e do papel das adesinas de superfície dos colonizadores iniciais Streptococcus oralis e Actinomyces naeslundii na formação e estruturação dos biofilmes de Candida albicans.
• Beneficiário: Indira Moraes Gomes Cavalcanti
• Modalidade de apoio: Bolsas no Brasil - Doutorado