Unraveling the role of high-intensity resistance training on left ventricle proteome: Is there a shift towards maladaptation?

Autor(es)

Dantas, Patricia Sousa ^[1] ; Sakata, Maisa Mayumi ^[1] ; Perez, Juliana Dineia ^[1] ; Harumi Watanabe, Regina Lucia ^[2] ; Bizerra, Fernando Cesar ^[3] ; das Neves, Vander Jose ^[4] ; Guzzoni, Vinicius ^[4] ; Marcondes, Fernanda Klein ^[4] ; Casarini, Dulce Elena ^[1] ; Cunha, Tatiana Sousa ^[5]

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Paulo UNIFESP, Dept Med, Discipline Nephrol, Sao Paulo, SP - Brazil

^[2] Fed Univ Sao Paulo UNIFESP, Dept Physiol, Discipline Nutr Physiol, Sao Paulo, SP - Brazil

^[3] Fed Univ Sao Paulo UNIFESP, Dept Med, Discipline Infect Dis, Special Mycol Lab LEMI, Sao Paulo, SP - Brazil

^[4] Univ Estadual Campinas, Fac Dent Piracicaba FOP UNICAMP, Dept Physiol Sci, Piracicaba, SP - Brazil

^[5] Fed Univ Sao Paulo UNIFESP, Inst Sci & Technol, Dept Sci & Technol, BR-12231280 Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Life Sciences; v. 152, p. 156-164, MAY 1 2016.
• Citações Web of Science: 7

Resumo

High-intensity resistance training (RT) induces adaptations that improve physiological function. However, high intensity, volume and/or frequency may lead to injury and other health issues such as adverse cardiac effects. The aim of this study was to evaluate the effect of RT on left ventricle proteome, and to identify the pathways involved on the harmful adaptations induced by this protocol. Male Wistar rats were randomized into 2 groups: Trained (T) and Sedentary (S). Animals from T group were trained for 6 weeks, and then all the animals were sacrificed and left ventricle was isolated for analysis. We identified 955 proteins, and 93 proteins were considered; 36 were expressed exclusively in T group, and 4 in S group. Based on quantitative analysis, 42 proteins were found overexpressed and 11 underexpressed in T group compared with S group. Using the Gene Ontology to relate the biological processes in which these proteins are involved, we conclude that RT protocol promotes changes similar to those found in the initial phase of heart failure, but we also observed a concomitant increased expression of protective proteins, suggesting the activation of pathways to avoid major damages on left ventricle and delay the onset of pathological hypertrophy. Statement of significance of the study: Our study shows that high-intensity RT protocol changes left ventricle proteome, modifying metabolic profile of heart tissue and inducing the expression of proteins that acts against cardiac injury. We hypothesize that these adaptations occur to prevent the onset of cardiac dysfunction. Despite highly significant, it remains to be determined whether these adaptations are sufficient to further keep left ventricle function and exert cardioprotection, and whether this panel will be shifted towards maladaptation, and heart failure. (c) 2016 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 02/05427-8 - Efeito de esteroide anabólico decanoato de nandrolona sobre a sensibilidade adrenérgica e sobre o metabolismo em ratos sedentários e treinados
• Beneficiário: Fernanda Klein Marcondes
• Modalidade de apoio: Auxílio à Pesquisa - Regular