Activation of myeloid dendritic cells, effector cells and regulatory T cells in lichen planus

Autor(es)

Domingues, Rosana ^[1] ; de Carvalho, Gabriel Costa ^[1] ; Aoki, Valeria ^[2] ; da Silva Duarte, Alberto Jose ^[1] ; Sato, Maria Notomi ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Trop Med Sao Paulo, Sch Med, Lab Dermatol & Immunodeficiencies, LIM 56, Dept Der, Av Dr Eneas de Carvalho Aguiar 500, 3rd Floor 24, BR-05403000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Med, Hosp Clin, Dermatol Outpatient Clin, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF TRANSLATIONAL MEDICINE; v. 14, JUN 10 2016.
• Citações Web of Science: 5

Resumo

Background: Lichen planus (LP) is a chronic mucocutaneous inflammatory disease. Evaluating the balance between regulatory T cells and effector T cells could be useful for monitoring the proinflammatory profile of LP. Therefore, this study aimed to assess populations of dendritic cells (DCs) and regulatory and effector T cells in peripheral blood samples collected from patients with LP to evaluate the polyfunctionality of T cells upon toll-like receptor (TLR) activation. Methods: Peripheral blood mononuclear cells collected from 18 patients with LP and 22 healthy control subjects were stimulated with agonists of TLR4, TLR7, TLR7/TLR8 or TLR9. Frequencies of circulating IFN-alpha(+) plasmacytoid DCs (pDCs); TNF-alpha(+) myeloid DCs (mDCs); regulatory T cells (Tregs); and IL-17-, IL-10-, IL-22-, TNF-, and IFN-gamma-secreting T cells were assessed via flow cytometry. Results: The frequencies of regulatory CD4(+) and CD8(+)CD25(+)Foxp3(+)CD127(low/-)T cells and TNF-alpha(+) mDCs were induced following activation with TLR4, TLR7 and TLR8 agonists in the LP group. Moreover, increased baseline frequencies of CD4(+)IL-10(+) T cells and CD8(+)IL-22(+) or IFN-gamma(+) T cells were found. In the LP group, TLR4 activation induced an increased frequency of CD4(+) IFN-gamma(+) T cells, while TLR7/8 and staphylococcal enterotoxin B (SEB) activation induced an increased frequency of CD8(+)IL-22(+) T cells. An increased frequency of polyfunctional CD4(+) T cells that simultaneously secreted 3 of the evaluated cytokines (not including IL-10) was verified upon TLR7/8/9 activation, while polyfunctional CD8(+) T cells were already detectable at baseline. Conclusions: TLR-mediated activation of the innate immune response induced the production of proinflammatory mDCs, Tregs and polyfunctional T cells in patients with LP. Therefore, TLR activation has an adjuvant role in inducing both innate and adaptive immune responses. (AU)

• Processo FAPESP: 11/20740-3 - Avaliação do perfil fenotípico e funcional de células dendríticas mielóides e plasmocitóides e dos linfócitos TCD4+ e TCD8+ no líquen plano
• Beneficiário: Maria Notomi Sato
• Modalidade de apoio: Auxílio à Pesquisa - Regular