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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Rapid turbidimetric assay for quantification of fusidic acid in a dermatological cream

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Autor(es):
Curbete, Mariane Machado [1] ; Nunes Salgado, Herida Regina [1]
Número total de Autores: 2
Afiliação do(s) autor(es):
[1] UNESP Univ Estadual Paulista, Sch Pharmaceut Sci, Dept Pharmaceut, Campus Araraquara, Rodovia Araraquara Jau, Km 1, BR-14800903 Sao Paulo - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Talanta; v. 153, p. 51-56, JUN 1 2016.
Citações Web of Science: 5
Resumo

Fusidic acid is an antibiotic steroid widely used for the treatment of serious infections caused by methicillin-resistant Staphylococcus aureus (MRSA) strains. Microbiological methods are indispensable to determine the mean percentage of antimicrobial in medicaments during manufacturing and quality control processes. The aim of this study was to develop and validate a microbiological method for the quantification of fusidic acid in dermatological cream by turbidimetry, using Staphylococcus epidermidis (ATCC 12228) and casoy broth as the culture medium. The validation parameters were in accordance with ICH specifications and demonstrated accuracy, precision, selectivity, and robustness, with linear ranges from 0.25 to 2.25 mu g mL(-1). This method is an alternative to the diffusion agar assay currently employed to quantify fusidic acid in dermatological cream, since it is sensitive; fast, and more economical. (C) 2016 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 13/02861-3 - Análise químico-farmacêutica do ácido fusídico em formulações de creme
Beneficiário:Mariane Machado Curbete
Linha de fomento: Bolsas no Brasil - Mestrado