Rapid turbidimetric assay for quantification of fusidic acid in a dermatological cream

Autor(es)

Curbete, Mariane Machado ^[1] ; Nunes Salgado, Herida Regina ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Sch Pharmaceut Sci, Dept Pharmaceut, Campus Araraquara, Rodovia Araraquara Jau, Km 1, BR-14800903 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Talanta; v. 153, p. 51-56, JUN 1 2016.
• Citações Web of Science: 5

Resumo

Fusidic acid is an antibiotic steroid widely used for the treatment of serious infections caused by methicillin-resistant Staphylococcus aureus (MRSA) strains. Microbiological methods are indispensable to determine the mean percentage of antimicrobial in medicaments during manufacturing and quality control processes. The aim of this study was to develop and validate a microbiological method for the quantification of fusidic acid in dermatological cream by turbidimetry, using Staphylococcus epidermidis (ATCC 12228) and casoy broth as the culture medium. The validation parameters were in accordance with ICH specifications and demonstrated accuracy, precision, selectivity, and robustness, with linear ranges from 0.25 to 2.25 mu g mL(-1). This method is an alternative to the diffusion agar assay currently employed to quantify fusidic acid in dermatological cream, since it is sensitive; fast, and more economical. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 13/02861-3 - Análise químico-farmacêutica do ácido fusídico em formulações de creme
• Beneficiário: Mariane Machado Curbete
• Modalidade de apoio: Bolsas no Brasil - Mestrado