Organization and comparative analysis of the mitochondrial genomes of bioluminescent Elateroidea (Coleoptera: Polyphaga)

Autor(es)

Amaral, Danilo T. ^{[1, 2]} ; Mitani, Yasuo ^[3] ; Ohmiya, Yoshihiro ^[4] ; Viviani, Vadim R. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Carlos UFSCar, Grad Sch Evolut Genet & Mol Biol, Sao Carlos, SP - Brazil

^[2] Grad Sch Biotechnol & Environm Monitoring UFSCar, Sorocaba, SP - Brazil

^[3] Natl Inst Adv Ind Sci & Technol, Bioprod Res Inst, Tsukuba, Ibaraki - Japan

^[4] Natl Inst Adv Ind Sci & Technol, Biomed Res Inst, Tsukuba, Ibaraki - Japan

• Tipo de documento: Artigo Científico
• Fonte: Gene; v. 586, n. 2, p. 254-262, JUL 25 2016.
• Citações Web of Science: 10

Resumo

Mitochondrial genome organization in the Elateroidea superfamily (Coleoptera), which include the main families of bioluminescent beetles, has been poorly studied and lacking information about Phengodidae family. We sequenced the mitochondrial genomes of Neotropical Lampyridae (Bicellonycha lividipennis), Phengodidae (Brasilocerus sp.2 and Phrixothrix hirtus) and Elateridae (Pyrearinus termitilluminans, Hapsodrilus ignifer and Teslasena femoralis). All species had a typical insect mitochondrial genome except for the following: in the elaterid T. femoralis genome there is a non-coding region between NADH2 and tRNA-Trp; in the phengodids Brasilocerus sp.2 and P. hirtus genomes we did not find the tRNA-Ile and tRNA-Gln. The P. hirtus genome showed a similar to 1.6 kb non-coding region, the rearrangement of tRNA-Tyr, a new tRNA-Leu copy, and several regions with higher AT contents. Phylogenetics analysis using Bayesian and ML models indicated that the Phengodidae + Rhagophthalmidae are closely related to Lampyridae family, and included Drilus flavescens (Drilidae) as an internal Glade within Elateridae. This is the first report that compares the mitochondria] genomes organization of the three main families of bioluminescent Elateroidea, including the first Neotropical Lampyridae and Phengodidae. The losses of tRNAs, and translocation and duplication events found in Phengodidae mt genomes, mainly in P. hirtus, may indicate different evolutionary rates in these mitochondrial genomes. The mitophylogenomics analysis indicates the monophyly of the three bioluminescent families and a closer relationship between Lampyridae and Phengodidae/Rhagophthalmidae, in contrast with previous molecular analysis. (C) 2016 Published by Elsevier B.V. (AU)

• Processo FAPESP: 11/19427-9 - Vagalumes da mata atlântica, cerrado e transição para Floresta Amazônica: biodiversidade, evolução molecular, e uso como bioindicadores
• Beneficiário: Vadim Viviani
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/00574-7 - Organização e análise comparativa do genoma mitocondrial para estudos filogenéticos em Elateroidea (Coleoptera: Polyphaga)
• Beneficiário: Danilo Trabuco do Amaral
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado Direto
• Processo FAPESP: 13/09594-0 - Análise dos transcriptomas das laternas, corpo gorduroso e túbulos de Malpighi de coleópteros, e suas relações com as origens da bioluminescência em Coleoptera e Diptera
• Beneficiário: Vadim Viviani
• Modalidade de apoio: Auxílio à Pesquisa - Regular