Kluyveromyces marxianus as a host for heterologous protein synthesis

Autor(es)

Gombert, Andreas K. ^[1] ; Madeira, Jr., Jose Valdo ^[1] ; Cerdan, Maria-Esperanza ^[2] ; Gonzalez-Siso, Maria-Isabel ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Food Engn, Rua Monteiro Lobato 80, BR-13083862 Campinas, SP - Brazil

^[2] Univ A Coruna, Fac Ciencias, Dept Bioloxia Celular & Mol, Grp EXPRELA, CICA, Campus Coruna, La Coruna 15071 - Spain

• Tipo de documento: Artigo de Revisão
• Fonte: Applied Microbiology and Biotechnology; v. 100, n. 14, p. 6193-6208, JUL 2016.
• Citações Web of Science: 9

Resumo

The preferentially respiring and thermotolerant yeast Kluyveromyces marxianus is an emerging host for heterologous protein synthesis, surpassing the traditional preferentially fermenting yeast Saccharomyces cerevisiae in some important aspects: K . marxianus can grow at temperatures 10 A degrees C higher than S. cerevisiae, which may result in decreased costs for cooling bioreactors and reduced contamination risk; has ability to metabolize a wider variety of sugars, such as lactose and xylose; is the fastest growing eukaryote described so far; and does not require special cultivation techniques (such as fed-batch) to avoid fermentative metabolism. All these advantages exist together with a high secretory capacity, performance of eukaryotic post-translational modifications, and with a generally regarded as safe (GRAS) status. In the last years, replication origins from several Kluyveromyces spp. have been used for the construction of episomal vectors, and also integrative strategies have been developed based on the tendency for non-homologous recombination displayed by K. marxianus. The recessive URA3 auxotrophic marker and the dominant Kan(R) are mostly used for selection of transformed cells, but other markers have been made available. Homologous and heterologous promoters and secretion signals have been characterized, with the K. marxianus INU1 expression and secretion system being of remarkable functionality. The efficient synthesis of roughly 50 heterologous proteins has been demonstrated, including one thermophilic enzyme. In this mini-review, we summarize the physiological characteristics of K. marxianus relevant for its use in the efficient synthesis of heterologous proteins, the efforts performed hitherto in the development of a molecular toolbox for this purpose, and some successful examples. (AU)

• Processo FAPESP: 15/14109-0 - Produção de etanol combustível pelo eucarioto mais rápido do planeta
• Beneficiário: Andreas Karoly Gombert
• Modalidade de apoio: Auxílio à Pesquisa - Regular