Photodynamic inactivation of a multispecies biofilm using curcumin and LED light

Autor(es)

Costa Quishida, Cristiane Campos ^[1] ; De Oliveira Mima, Ewerton Garcia ^[2] ; Jorge, Janaina Habib ^[2] ; Vergani, Carlos Eduardo ^[2] ; Bagnato, Vanderlei Salvador ^[3] ; Pavarina, Ana Claudia ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Dept Dent Mat & Prosthodont, Inst Sci & Technol, Sch Dent, Ave Engn Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[2] Univ Estadual Paulista, UNESP, Araraquara Dent Sch, Dept Dent Mat & Prosthodont, Rua Humaita 1680, BR-14801903 Araraquara, SP - Brazil

^[3] Univ Sao Paulo, Phys Inst Sao Carlos, Ave Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 31, n. 5, p. 997-1009, JUL 2016.
• Citações Web of Science: 13

Resumo

This study evaluated the potential of curcumin-mediated antimicrobial photodynamic inactivation (API) on multispecies biofilms of Candida albicans, Candida glabrata, and Streptococcus mutans of different ages. Acrylic samples (n = 480) were made with standardized rough surfaces and incubated with bacteria and yeast for 24 or 48 h. API was performed with curcumin (80, 100, 120 mu M) and LED light. Additional acrylic samples were treated with curcumin or LED light only. Positive control samples received neither light nor curcumin. After API, colony counts were quantified (CFU/mL), cell metabolism was determined by means of XTT assay, and the total biofilm biomass was evaluated using Crystal Violet (CV) staining assay and images were obtained by confocal laser scanning microscopy (CLSM). The data were analyzed by nonparametric two-way ANOVA and post hoc Tukey tests (alpha < 0.05). For 24-h biofilm, API resulted in statistically significant difference (rho < 0.001) of viability of C. albicans compared with control (P-L-) for all Cur concentrations. For 48-h biofilm, API resulted in statistically significant difference (rho < 0.001) compared with control only when Cur at 120 mu M was used. API promoted statistically significant difference (rho <= 0.001) in the viability of S. mutans and C. glabrata for all Cur concentrations in the two biofilm ages. In addition, API produced a statistically significant difference (rho < 0.001) of metabolic activity and of total biomass (rho < 0.001) of multispecies biofilms compared with control for all Cur concentrations. It can be concluded that both 24- and 48-h biofilms were susceptible to API mediated by Cur; however, 24-h biofilm was more sensitive than the 48-h biofilm. (AU)

• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs