Antifungal activity of plant-derived essential oils on Candida tropicalis planktonic and biofilms cells

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Cury Souza, Caio Marcelo ^[1] ; Pereira Junior, Silvio Alves ^[1] ; Moraes, Thais da Silva ^[1] ; Damasceno, Jaqueline Lopes ^[1] ; Mendes, Suzana Amorim ^[1] ; Dias, Herbert Junior ^[2] ; Stefani, Ricardo ^[3] ; Tavares, Denise Crispim ^[1] ; Gomes Martins, Carlos Henrique ^[1] ; Miller Crotti, Antonio Eduardo ^[2] ; Soares Mendes-Giannini, Maria Jose ^[4] ; Pires, Regina Helena ^[4]

Afiliação do(s) autor(es)

^[1] Univ Franca, Lab Pesquisa Microbiol Aplicada, Franca, SP - Brazil

^[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Sao Paulo - Brazil

^[3] Univ Fed Mato Grosso, Inst Ciencias Exatas & Terra, Pontal Do Araguaia, MT - Brazil

^[4] Univ Estadual Paulista, Fac Ciencias Farmaceut, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Medical Mycology; v. 54, n. 5, p. 515-523, JUL 2016.
• Citações Web of Science: 16

Resumo

Dental prosthesis supports Candida species growth and may predispose the oral cavity to lesions. C. tropicalis has emerged as a colonizer of prosthesis and has shown resistance to clinically used antifungal agents, which has increased the search for new antifungals. This work describes the effectiveness of fifteen essential oils (EOs) against C. tropicalis. The EOs were obtained by hydrodistillation and were chemically characterized by gas chromatography-mass spectrometry. The antifungal activities of the EOs were evaluated by the microdilution method and showed that Pelargonium graveolens (Geraniaceae) (PG-EO) was the most effective oil. Geraniol and linalool were the major constituents of PG-EO. The 2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide (XTT) assay showed that all the clinical C. tropicalis strains formed viable biofilms. Scanning electron microscopy examination of the biofilms revealed a complex architecture with basal layer of yeast cells and an upper layer of filamentous cells. Treatments with PG-EO, linalool, and geraniol significantly reduced the number of viable biofilm cells and inhibited biofilm formation after exposure for 48 h. PG-EO, geraniol, and linalool were not toxic to normal human lung fibroblasts (GM07492A) at the concentrations they were active against C. tropicalis. Together, our results indicated that C. tropicalis is susceptible to treatment with PG-EO, geraniol, and linalool, which could become options to prevent or treat this infection. (AU)

• Processo FAPESP: 11/12734-3 - Candida parapsilosis e C. orthopsilosis isoladas de águas de hemodiálise. Aplicação de metodologias proteomicas e de fotocatálise.
• Beneficiário: Regina Helena Pires
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 07/54241-8 - Prospecção de óleos essenciais de espécies brasileiras cultiváveis com atividade contra patógenos da cavidade bucal
• Beneficiário: Antônio Eduardo Miller Crotti
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Jovens Pesquisadores