Multimodal and Non-Linear Optical Microscopy Applications in Reproductive Biology

Autor(es)

Adur, J. ^{[1, 2, 3]} ; Barbosa, G. O. ^[4] ; Pelegati, V. B. ^{[5, 1]} ; Baratti, M. O. ^[5] ; Cesar, C. L. ^{[5, 1, 6]} ; Casco, V. H. ^{[2, 3]} ; Carvalho, H. F. ^{[4, 5]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Phys Gleb Wataghin, Biophoton Grp, Opt & Photon Res Ctr CEPOF, Campinas, SP - Brazil

^[2] CONICET UNER, CITER, ByPIB, Concepcion Del Uruguay - Argentina

^[3] Natl Univ Entre Rios, Sch Bioengn, Microscopy Lab Appl Mol & Cellular Studies, Concepcion Del Uruguay - Argentina

^[4] Univ Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, Campinas, SP - Brazil

^[5] INFABiC Natl Inst Sci & Technol Photon Appl Cell, Campinas, SP - Brazil

^[6] Fed Univ Ceara UFC, Dept Phys, Fortaleza, Ceara - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: MICROSCOPY RESEARCH AND TECHNIQUE; v. 79, n. 7, p. 567-582, JUL 2016.
• Citações Web of Science: 2

Resumo

A plethora of optical techniques is currently available to obtain non-destructive, contactless, real time information with subcellular spatial resolution to observe cell processes. Each technique has its own unique features for imaging and for obtaining certain biological information. However none of the available techniques can be of universal use. For a comprehensive investigation of biological specimens and events, one needs to use a combination of bioimaging methods, often at the same time. Some modern confocal/multiphoton microscopes provide simultaneous fluorescence, fluorescence lifetime imaging, and four-dimensional imaging. Some of them can also easily be adapted for harmonic generation imaging, and to permit cell manipulation technique. In this work we present a multimodal optical workstation that extends a commercially available confocal microscope to include nonlinear/multiphoton microscopy and optical manipulation/stimulation tools. The nonlinear microscopy capabilities were added to the commercial confocal microscope by exploiting all the flexibility offered by the manufacturer. The various capabilities of this workstation as applied directly to reproductive biology are discussed. (C) 2016 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 11/51591-3 - Javier Fernando Adur | Universidad Nacional de Entre Rios - Argentina
• Beneficiário: Carlos Lenz Cesar
• Linha de fomento: Auxílio à Pesquisa - Pesquisador Visitante - Internacional
• Processo FAPESP: 09/16150-6 - Regulação androgênica, sinalização e interações celulares no desenvolvimento, fisiologia e regressão prostática
• Beneficiário: Hernandes Faustino de Carvalho
• Linha de fomento: Auxílio à Pesquisa - Temático