Molecular immune response of channel catfish immunized with live theronts of Ichthyophthirius multifiliis

Autor(es)

Xu, De-Hai ^[1] ; Zhang, Qi-Zhong ^[2] ; Shoemaker, Craig A. ^[1] ; Zhang, Dunhua ^[1] ; Moreira, Gabriel S. A. ^[3]

Afiliação do(s) autor(es)

^[1] ARS, USDA, Aquat Anim Hlth Res Unit, 990 Wire Rd, Auburn, AL 36832 - USA

^[2] Jinan Univ, Inst Hydrobiol, Key Lab Eutrophicat & Red Tide Prevent, Guangdong Higher Educ Inst, Guangzhou 510632, Guangdong - Peoples R China

^[3] Univ Sao Paulo, Coll Anim Sci & Food Engn, Parasitol Lab, Av Duque Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FISH & SHELLFISH IMMUNOLOGY; v. 54, p. 86-92, JUL 2016.
• Citações Web of Science: 7

Resumo

The parasite Ichthyophthirius multifiliis (Ich) has been reported in various freshwater fishes worldwide and results in severe losses to both food and aquarium fish production. The fish surviving natural infections or immunized with live theronts develop strong specific and non-specific immune responses. Little is known about how these immune genes are induced or how they interact and lead to specific immunity against Ichthyophthirius multifiliis in channel catfish Ictalurus punctatus. This study evaluated the differential expression of immune-related genes, including immunoglobulin, immune cell receptor, cytokine, complement factor and toll-like receptors in head kidney from channel catfish at different time points after immunization with live theronts of I. multifiliis. The immunized fish showed significantly higher anti-Ich antibody expressed as immobilization titer and ELISA titer than those of control fish. The vast majority of immunized fish (95%) survived theront challenge. Expression of IgM and IgD heavy chain genes exhibited a rapid increase from 4 hour (h4) to 2 days (d2) post immunization. Expression of immune cell receptor genes (CD4, CD8-alpha, MHC I, MHC II beta, TcR-alpha, and TcR-beta) showed up-regulation from h4 to d6 post immunization, indicating that different immune cells were actively involved in cellular immune response. Cytokine gene expression (IL-1 beta a, IL-1 beta b, IFN-gamma and TNF-alpha) increased rapidly at h4 post immunization and were at an up-regulated level until d2 compared to the bovine serum albumin control. Expression of complement factor and toll-like receptor genes exhibited a rapid increase from h4 to d2 post immunization. Results of this study demonstrated differential expression of genes involved in the specific or non-specific immune response post immunization and that the vaccination against Ich resulted in protection against infection by I. multifiliis. Published by Elsevier Ltd. (AU)

• Processo FAPESP: 15/01822-0 - Comparação da expressão de genes imunológicos do peixe Ictalurus punctatus mantido em diferentes temperaturas após infecção com o parasita Ichthyophthirius multifiliis
• Beneficiário: Gabriel Sassarão Alves Moreira
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado