Long-term in vivo polychlorinated biphenyl 126 exposure induces oxidative stress and alters proteomic profile on islets of Langerhans

Autor(es)

Loiola, Rodrigo Azevedo ^[1] ; dos Anjos, Fabyana Maria ^[1] ; Shimada, Ana Lucia ^[1] ; Cruz, Wesley Soares ^[1] ; Drewes, Carine Cristiane ^[1] ; Rodrigues, Stephen Fernandes ^[1] ; Cardozo, Karina Helena Morais ^[2] ; Carvalho, Valdemir Melechco ^[2] ; Pinto, Ernani ^[1] ; Farsky, Sandra Helena ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin & Toxicol Analyses, Sao Paulo - Brazil

^[2] Fleury Grp, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 6, JUN 13 2016.
• Citações Web of Science: 6

Resumo

It has been recently proposed that exposure to polychlorinated biphenyls (PCBs) is a risk factor to type 2 diabetes mellitus (DM2). We investigated this hypothesis using long-term in vivo PCB126 exposure to rats addressing metabolic, cellular and proteomic parameters. Male Wistar rats were exposed to PCB126 (0.1, 1 or 10 mu g/kg of body weight/ day; for 15 days) or vehicle by intranasal instillation. Systemic alterations were quantified by body weight, insulin and glucose tolerance, and blood biochemical profile. Pancreatic toxicity was measured by inflammatory parameters, cell viability and cycle, free radical generation, and proteomic profile on islets of Langerhans. In vivo PCB126 exposure enhanced the body weight gain, impaired insulin sensitivity, reduced adipose tissue deposit, and elevated serum triglycerides, cholesterol, and insulin levels. Inflammatory parameters in the pancreas and cell morphology, viability and cycle were not altered in islets of Langerhans. Nevertheless, in vivo PCB126 exposure increased free radical generation and modified the expression of proteins related to oxidative stress on islets of Langerhans, which are indicative of early beta-cell failure. Data herein obtained show that long-term in vivo PCB126 exposure through intranasal route induced alterations on islets of Langerhans related to early end points of DM2. (AU)

• Processo FAPESP: 12/02994-0 - Estudo experimental da exposição ao PCB126 sobre a indução de Diabetes mellitus tipo II
• Beneficiário: Sandra Helena Poliselli Farsky
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/09677-8 - Estudo experimental da exposição ao PCB126 sobre a indução de Diabetes Mellitus tipo II
• Beneficiário: Ana Lucia Borges Shimada
• Modalidade de apoio: Bolsas no Brasil - Doutorado