Human bronchial epithelial cells exposed in vitro to diesel exhaust particles exhibit alterations in cell rheology and cytotoxicity associated with decrease in antioxidant defenses and imbalance in pro- and anti-apoptotic gene expression

Autor(es):Mostrar menos -

Seriani, Robson ^{[1, 2]} ; Carvalho de Souza, Claudia Emanuele ^[3] ; Krempel, Paloma Gava ^[4] ; Frias, Daniela Perroni ^[1] ; Matsuda, Monique ^[4] ; Correia, Aristides Tadeu ^[5] ; Justo Ferreira, Marcia Zotti ^[6] ; Alencar, Adriano Mesquita ^[6] ; Negri, Elnara Marcia ^[1] ; Nascimento Saldiva, Paulo Hilario ^[1] ; Mauad, Thais ^[1] ; Macchione, Mariangela ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Lab Expt Air Pollut LIM05, Dept Pathol, Sch Med, Av Dr Arnaldo 455, 1 Andar, Sala 1150, BR-CEP 0124 Sao Paulo, SP - Brazil

^[2] FAM Fac Amer, Rua Augusta, 1508, 3 Andar, BR-01304001 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Inst Biosci, Dept Physiol, Lab Chronopharmacol, Rua Matao Travessa 14, Cidade Univ, BR-05508900 Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Lab Invest Ophthalmol LIM 33, Sch Med, Dr Arnaldo 455, 5 Andar, BR-01246903 Sao Paulo, SP - Brazil

^[5] Univ Sao Paulo, Heart Inst InCor, Dept Cardiopulmonol, Sch Med, Av Dr Eneas Carvalho Aguiar, 44, BR-05403000 Sao Paulo, SP - Brazil

^[6] Univ Sao Paulo, Lab Microrheol & Mol Physiol, Inst Phys, Rua Matao, Travessa R Numero 187, Cidade Univ, BR-05508090 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Environmental Science and Pollution Research; v. 23, n. 10, p. 9862-9870, MAY 2016.
• Citações Web of Science: 10

Resumo

Diesel exhaust particles (DEPs) from diesel engines produce adverse alterations in cells of the airways by activating intracellular signaling pathways and apoptotic gene overexpression, and also by influencing metabolism and cytoskeleton changes. This study used human bronchial epithelium cells (BEAS-2B) in culture and evaluates their exposure to DEPs (15ug/mL for 1 and 2 h) in order to determine changes to cell rheology (viscoelasticity) and gene expression of the enzymes involved in oxidative stress, apoptosis, and cytotoxicity. BEAS-2B cells exposed to DEPs were found to have a significant loss in stiffness, membrane stability, and mitochondrial activity. The genes involved in apoptosis {[}B cell lymphoma 2 (BCL-2 and caspase-3)] presented inversely proportional expressions (p=0.05, p=0.01, respectively), low expression of the genes involved in antioxidant responses {[}SOD1 (superoxide dismutase 1); SOD2 (superoxide dismutase 2), and GPx (glutathione peroxidase) (p=0.01)], along with an increase in cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1) (p=0.01). These results suggest that alterations in cell rheology and cytotoxicity could be associated with oxidative stress and imbalance between pro-and antiapoptotic genes. (AU)

• Processo FAPESP: 11/50334-7 - Avaliação da toxicidade do material particulado urbano em cultura de células do epitélio brônquico humano: estudo da expressão gênica de enzimas antioxidante e sinalização celular
• Beneficiário: Robson Seriani
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto