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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

MicroRNA-10 modulates Hox genes expression during Nile tilapia embryonic development

Texto completo
Autor(es):
Giusti, Juliana [1] ; Pinhal, Danillo [2] ; Moxon, Simon [3] ; Campos, Camila Lovaglio [2] ; Munsterberg, Andrea [4] ; Martins, Cesar [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ, Dept Morphol, Inst Biosci, Botucatu, SP - Brazil
[2] Sao Paulo State Univ, Dept Genet, Inst Biosci, Botucatu, SP - Brazil
[3] Genome Anal Ctr, Norwich Res Pk, Norwich, Norfolk - England
[4] Univ E Anglia, Sch Biol Sci, Norwich Res Pk, Norwich NR4 7TJ, Norfolk - England
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: MECHANISMS OF DEVELOPMENT; v. 140, p. 12-18, MAY 2016.
Citações Web of Science: 7
Resumo

Hox gene clusters encode a family of transcription factors that govern anterior-posterior axis patterning during embryogenesis in all bilaterian animals. The time and place of Hox gene expression are largely determined by the relative position of each gene within its cluster. Furthermore, Hox genes were shown to have their expression fine-tuned by regulatory microRNAs (miRNAs). However, the mechanisms of miRNA-mediated regulation of these transcription factors during fish early development remain largely unknown. Here we have profiled three highly expressed miR-10 family members of Nile tilapia at early embryonic development, determined their genomic organization as well as performed functional experiments for validation of target genes. Quantitative analysis during developmental stages showed miR-10 family expression negatively correlates with the expression of HoxA3a, HoxB3a and HoxD10a genes, as expected for bona fide miRNA-mRNA interactions. Moreover, luciferase assays demonstrated that HoxB3a and HoxD10a are targeted by miR-10b-5p. Overall, our data indicate that the miR-10 family directly regulates members of the Hox gene family during Nile tilapia embryogenesis. (C) 2016 Elsevier Ireland Ltd. All rights reserved. (AU)

Processo FAPESP: 11/06465-0 - Identificação e caracterização de microRNAs no genoma da tilápia do Nilo Oreochromis niloticus
Beneficiário:César Martins
Modalidade de apoio: Auxílio à Pesquisa - Regular