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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Rapid detection of infectious rotavirus group A using a molecular beacon assay

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Autor(es):
Bertol, Jessica Wildgrube [1] ; Viccari Gatti, Maria Silvia [1]
Número total de Autores: 2
Afiliação do(s) autor(es):
[1] State Univ Campinas UNICAMP, Inst Biol, Dept Genet Evolut & Bioagents, BR-13083970 Campinas, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Journal of Virological Methods; v. 234, p. 156-159, AUG 2016.
Citações Web of Science: 0
Resumo

Rapid, sensitive and specific methods are necessary to detect and quantify infectious viruses. Cultivating and detecting enteric viruses in cell culture are difficult, thus impairing the advancement of knowledge regarding virus -induced diarrhea. Rotavirus (RV) detection has been conducted by serological or molecular biology methods, which do not provide information regarding viral infectivity. Molecular beacons (MBs) have demonstrated efficacy for viral detection in cell culture. We propose a MB assay to detect human rotavirus group A (HuRVA) in cell culture. MA104 cells were mock -infected or infected with HuRVA strains (RotaTeq vaccine and KS strains), and a specific MB for the HuRVA VP6 gene was used for virus detection. Mock -infected cells showed basal fluorescence, while infected cells exhibited increased fluorescence emission. MB hybridization to the viral mRNA target of HuRVA was confirmed. Fluorescence increased according to the increase in the number of infectious viral particles per cell (MOI 0.5-MOI 1). This technique provides quick and efficient HuRVA detection in cell culture without a need for viral culture for several days or many times until cytopathic effects are visualized. This methodology could be applied in the selection of samples for developing RV vaccines. (C) 2016 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 11/23865-1 - Estudo da ação de NSP4 de rotavírus sobre a permeabilidade celular in vitro e o acúmulo de fluidos intestinais em ratos (modelo in vivo)
Beneficiário:Jéssica Wildgrube Bertol
Modalidade de apoio: Bolsas no Brasil - Doutorado