Effect of the environment on the secondary metabolic profile of Tithonia diversifolia: a model for environmental metabolomics of plants

Autor(es)

Sampaio, Bruno Leite ^{[1, 2]} ; Edrada-Ebel, RuAngelie ^[1] ; Da Costa, Fernando Batista ^[2]

Afiliação do(s) autor(es)

^[1] Univ Strathclyde, Strathclyde Inst Pharm & Biomed Sci, 161 Cathedral St, Glasgow G4 0RE, Lanark - Scotland

^[2] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Lab Pharmacognosy, AsterBioChem Team, Av Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 6, JUL 7 2016.
• Citações Web of Science: 71

Resumo

Tithonia diversifolia is an invasive weed commonly found in tropical ecosystems. In this work, we investigate the influence of different abiotic environmental factors on the plant's metabolite profile by multivariate statistical analyses of spectral data deduced by UHPLC-DAD-ESI-HRMS and NMR methods. Different plant part samples of T. diversifolia which included leaves, stems, roots, and inflorescences were collected from two Brazilian states throughout a 24-month period, along with the corresponding monthly environmental data. A metabolomic approach employing concatenated LC-MS and NMR data was utilised for the first time to study the relationships between environment and plant metabolism. A seasonal pattern was observed for the occurrence of metabolites that included sugars, sesquiterpenes lactones and phenolics in the leaf and stem parts, which can be correlated to the amount of rainfall and changes in temperature. The distribution of the metabolites in the inflorescence and root parts were mainly affected by variation of some soil nutrients such as Ca, Mg, P, K and Cu. We highlight the environment-metabolism relationship for T. diversifolia and the combined analytical approach to obtain reliable data that contributed to a holistic understanding of the influence of abiotic environmental factors on the production of metabolites in various plant parts. (AU)

• Processo FAPESP: 11/13361-6 - Avaliação da variação sazonal dos principais metabólitos secundários e da atividade anti-inflamatória de extratos de Tithonia diversifolia (Hemsl.) A. Gray (Asteraceae)
• Beneficiário: Bruno Leite Sampaio
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/51454-3 - Estudos morfoanatômicos, metabolômicos e moleculares como subsídios à sistemática de espécies de Asteraceae e acesso ao seu potencial farmacológico
• Beneficiário: Beatriz Appezzato da Glória
• Modalidade de apoio: Auxílio à Pesquisa - Temático