Alkaloids and biological activity of beribá (Annona hypoglauca)

Autor(es)

Maria V.N. Rinaldi ^[1] ; Ingrit E.C. Díaz ^[2] ; Ivana B. Suffredini ^[3] ; Paulo R.H. Moreno ^[4]

Afiliação do(s) autor(es)

^[1] Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Programa de Pós-graduação em Fármaco e Medicamento - Brasil

^[2] Universidade Paulista. Laboratório de Extração. Núcleo de Pesquisas em Biodiversidade - Brasil

^[3] Universidade Paulista. Laboratório de Extração. Núcleo de Pesquisas em Biodiversidade - Brasil

^[4] Universidade de São Paulo. Instituto de Química - Brasil

• Tipo de documento: Artigo Científico
• Fonte: REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY; v. 27, n. 1, p. 77-83, 2017-02-00.

Resumo

ABSTRACT Annona hypoglauca Mart., Annonaceae, popularly known as “beribá”, was collected in flooded areas of the Amazonian Rain Forest. The crude extract obtained from this species was found to be cytotoxic against human cancer cells. Chemical information on A. hypoglauca is scarce. So, the present work aimed the isolation and identification of its alkaloids and to test their cytotoxic activity. Alkaloids were obtained from stem by acid–base partitioning and the remaining alkaloid-free extract was partitioned with organic solvents. Gas chromatography–mass spectrometry GC/MS analysis of total alkaloids allowed the identification of four aporphine alkaloids: actinodaphnine, anonaine, isoboldine and nornuciferine. Total alkaloids were fractionated by column chromatography and were purified by preparative thin-layer-chromatography, which allowed the isolation of two aporphine alkaloids, actinodaphnine and isoboldine, characterized by NMR and CG–MS analyses. This is the first report for the occurrence of actinodaphnine in Annona species. All the samples were tested in cytotoxic and antibacterial assays. Total alkaloid extract and its fractions showed antimicrobial activity against Staphylococcus aureus and Enterococcus faecalis. In the cytotoxicity assay, the crude extract showed a lethal effect against breast and colon cancer cells. Isoboldine-containing FA5 and actinodaphnine-containing FA6 showed activity against breast cancer cell line, while the alkaloid-free fractions did not show significant activity against cancer cell lines. (AU)

• Processo FAPESP: 99/05904-6 - Avaliação da resposta citotóxica, antitumoral e antimicrobiana in vitro e in vivo de extratos de algumas espécies vegetais pertencentes às famílias Apocynaceae, Annonaceae, Clusiaceae e Euphorbiaceae
• Beneficiário: Riad Naim Younes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/58706-8 - Avaliação da atividade antitumoral, antibacteriana e ação sobre o sistema nervoso central e hormônios de extratos obtidos de plantas amazônicas e da Mata Atlântica
• Beneficiário: Ivana Barbosa Suffredini
• Modalidade de apoio: Auxílio à Pesquisa - Regular