Effect of ultrasonic streaming on infra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment

Autor(es)

Marcela Paola Castro ARIAS ; Amanda Garcia Alves MALIZA ; Raquel Zanin MIDENA ; Márcia Sirlene Zardin GRAEFF ; Marco Antonio Húngaro DUARTE ; Flaviana Bombarda de ANDRADE

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Oral Science; v. 24, n. 6, p. 575-581, Dez. 2016.
• Citações Web of Science: 5

Resumo

ABSTRACT Objective The antimicrobial effect of ultrasonic agitation of calcium hydroxide (CH) pastes in infected bovine dentin and their penetrability were evaluated using confocal laser scanning microscopy (CLSM) and microbiological culture. Material and Methods Fifty-two bovine teeth were infected with Enterococcus faecalis using a new contamination protocol; then they received CH paste and were divided into groups with or without ultrasound. Ultrasonic agitation was conducted for 1 min with a plain point insert. After 15 d, the CLSM analyzed the viable and dead bacteria with Live and Dead assay. The dentinal wall debris was collected by burs, and the colony forming units (CFU/mL) were counted. The penetrability of the paste inside dentinal tubules was tested using the B-rodamine dye. Results The calcium hydroxide paste showed better results with the use of ultrasonic agitation (p<0.05). Conclusion The ultrasonic agitation of CH paste increased its antimicrobial action and was responsible for intradentinal penetration with the fulfilment of the tubules. (AU)

• Processo FAPESP: 10/20186-3 - Fatores de adesão e biofilmes gerados por micro-organismos isolados de canais radiculares
• Beneficiário: Flaviana Bombarda de Andrade
• Modalidade de apoio: Auxílio à Pesquisa - Regular