| Texto completo | |
| Autor(es): |
Galeano, E.
;
Barroso, A. A. M.
;
Vasconcelos, T. S.
;
Lopez-Rubio, A.
;
Albrecht, A. J. P.
;
Victoria Filho, R.
;
Carrer, H.
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Genetics and Molecular Research; v. 15, n. 3 2016. |
| Citações Web of Science: | 0 |
| Resumo | |
Weed resistance to herbicides is a natural phenomenon that exerts selection on individuals in a population. In Brazil, glyphosate resistance was recently detected in Digitaria insularis. The objective of this study was to elucidate mechanisms of weed resistance in this plant, including genetic variability, allelism, amino acid substitutions, gene expression, and enzymatic activity levels. Most of these have not previously been studied in this species. D. insularis DNA sequences were used to analyze genetic variability. cDNA from resistant and susceptible plants was used to identify mutations, alleles, and 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) expression, using real-time quantitative reverse transcription-polymerase chain reaction. In addition, EPSPS activity was measured. We found a decrease in genetic variability between populations related to glyphosate application. Substitutions from proline to threonine and tyrosine to cysteine led to a decrease in EPSPS affinity for the glyphosate. In addition, the EPSPS enzymatic activity was slightly higher in resistant plants, whereas EPSPS gene expression was almost identical in both biotypes, suggesting feedback regulation at different levels. To conclude, our results suggest new molecular mechanisms used by D. insularis to increase glyphosate resistance. (AU) | |
| Processo FAPESP: | 12/10371-3 - Comportamento da soja rr em distintos ambientes de produção, submetida a diferentes manejos, formulações e doses de glyphosate |
| Beneficiário: | Alfredo Junior Paiola Albrecht |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 13/06299-8 - Análise do perfil transcripcional de tecidos lignificados em Tectona grandis L. f |
| Beneficiário: | Esteban Galeano Gómez |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 12/10372-0 - Caracterização da superfície foliar e genética de Digitaria insularis (L.) Fedde e eficácia de controle de associações de herbicidas |
| Beneficiário: | Arthur Arrobas Martins Barroso |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 10/19294-6 - Caracterização da família gênica CAD envolvida em tecidos de formação de madeira em Tectona grandis |
| Beneficiário: | Tarcísio Sales Vasconcelos |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |