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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)


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Ditondo Micas, Andre Fernando ; Ferreira, Germano Aguiar ; Laure, Helen Julie ; Rosa, Jose Cesar ; Gentile Bitondi, Marcia Maria
Número total de Autores: 5
Tipo de documento: Artigo Científico
Fonte: Archives of Insect Biochemistry and Physiology; v. 93, n. 1, p. 3-24, SEP 2016.
Citações Web of Science: 4

The integument of insects and other arthropods is composed of an inner basal lamina coated by the epidermis, which secretes the bulk of the outer integument layer, the cuticle. The genome sequencing of several insect species has allowed predicting classes of proteins integrating the cuticle. However, only a small proportion of them, as well as other proteins in the integumentary system, have been validated. Using two-dimensional gel electrophoresis coupled with mass spectrometry, we identified 45 different proteins in a total of 112 selected gel spots derived from thoracic integument samples of developing honeybee workers, including 14 cuticular proteins (AmelCPR 3, AmelCPR 12, AmelCPR 16, AmelCPR 27, apidermin 2, apidermin 3, endocuticle structural glycoprotein SgAbd-8-like, LOC100577363, LOC408365, LOC413679, LOC725454, LOC100576916, LOC725838, and peritrophin 3-C analogous). Gene ontology functional analysis revealed that the higher proportions of the identified proteins have molecular functions related to catalytic and structural molecule activities, are involved in metabolic biological processes, and pertain to the protein class of structural or cytoskeletal proteins and hydrolases. It is noteworthy that 26.7% of the identified proteins, including five cuticular proteins, were revealed as protein species resulting from allelic isoforms or derived from posttranslational modifications. Also, 66.7% of the identified cuticular proteins were expressed in more than one developmental phase, thus indicating that they are part of the larval, pupal, and adult cuticle. Our data provide experimental support for predicted honeybee gene products and new information on proteins expressed in the developing integument. (C) 2016 Wiley Periodicals, Inc. (AU)

Processo FAPESP: 14/13136-0 - RNA-seq para caracterização da expressão gênica relacionada à construção e diferenciação do exoesqueleto de insetos utilizando abelhas como modelo
Beneficiário:Marcia Maria Gentile Bitondi
Linha de fomento: Auxílio à Pesquisa - Regular