| Texto completo | |
| Autor(es): |
Lopes, Joao Henrique
;
Magalhae, Jessica Aparecida
;
Gouueia, Rubia Figueredo
;
Bertran, Celso Aparecido
;
Motisuke, Mariana
;
Camargo, Samira E. A.
;
Triches, Eliandra de Sousa
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS; v. 62, p. 10-23, SEP 2016. |
| Citações Web of Science: | 16 |
| Resumo | |
This paper investigates the microstructure and the mechanical properties of beta-tricalcium phosphate (beta-TCP) three-dimensional (3D) porous materials reinforced with 45S5 bioactive glass (BG). beta-TCP and beta-TCP/x%-BG scaffolds with interconnected pores networks, suitable for bone regeneration, were fabricated by gel-casting method. Mechanical properties, porosity, and morphological characteristics were evaluated by compressive strength test, scanning electron microscopy (SEM) and X-ray micro tomography analysis, whereas the structures were fully explored by XRD, and Raman spectroscopy. To the best of our knowledge, this is the first time where the mechanism for understanding the effect of bioglass on the mechanical properties and microstruture of beta-TCP/45S5-BG scaffolds has been systematically studied. The findings showed that ionic product lixiviated from 45S5 bioactive glass, rich in silicon species and sodium ion, catalyzes a phase transition from beta-TCP to Si-TCP by replacement of phosphorus for silicon and contributes to the improvement of scaffolds mechanical properties. The compressive strength of beta-TCP/5%-BG and beta-TCP/7.5%-BG was improved around 200% in comparison to pure beta-TCP. Osteoblast-like cells (MG 63) were exposed to the materials for 24 h through the use of medium conditioned by beta-tricalcium phosphate/bioactive glass. Cell viability was measured by MTT assay in the cells and the data obtained were submitted to ANOVA, Tukey's multiple comparison (p<0.05). The beta-TCP/7.5-BG promoted an increase of cell proliferation. The results suggest that compositions and processing method studied may provide appropriate materials for tissue engineering. (C) 2016 Elsevier Ltd. All rights reserved. (AU) | |
| Processo FAPESP: | 10/00863-0 - Processamento e caracterização de suportes porosos nanoestruturados do tipo B-TCP/Al2O3 para aplicações biomédicas |
| Beneficiário: | Eliandra de Sousa Trichês |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 11/09240-9 - Otimização de cimentos de fosfato de cálcio para aplicação na ortopedia e na engenharia tecidual |
| Beneficiário: | Mariana Motisuke |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 11/17877-7 - Desenvolvimento de novos scaffolds poliméricos por eletrofiação com incorporação de nanotubos alinhados e nanohidroxiapatita para regeneração óssea |
| Beneficiário: | Anderson de Oliveira Lobo |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |