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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Hierarchical structures of beta-TCP/45S5 bioglass hybrid scaffolds prepared by gelcasting

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Lopes, Joao Henrique ; Magalhae, Jessica Aparecida ; Gouueia, Rubia Figueredo ; Bertran, Celso Aparecido ; Motisuke, Mariana ; Camargo, Samira E. A. ; Triches, Eliandra de Sousa
Total Authors: 7
Document type: Journal article
Web of Science Citations: 16

This paper investigates the microstructure and the mechanical properties of beta-tricalcium phosphate (beta-TCP) three-dimensional (3D) porous materials reinforced with 45S5 bioactive glass (BG). beta-TCP and beta-TCP/x%-BG scaffolds with interconnected pores networks, suitable for bone regeneration, were fabricated by gel-casting method. Mechanical properties, porosity, and morphological characteristics were evaluated by compressive strength test, scanning electron microscopy (SEM) and X-ray micro tomography analysis, whereas the structures were fully explored by XRD, and Raman spectroscopy. To the best of our knowledge, this is the first time where the mechanism for understanding the effect of bioglass on the mechanical properties and microstruture of beta-TCP/45S5-BG scaffolds has been systematically studied. The findings showed that ionic product lixiviated from 45S5 bioactive glass, rich in silicon species and sodium ion, catalyzes a phase transition from beta-TCP to Si-TCP by replacement of phosphorus for silicon and contributes to the improvement of scaffolds mechanical properties. The compressive strength of beta-TCP/5%-BG and beta-TCP/7.5%-BG was improved around 200% in comparison to pure beta-TCP. Osteoblast-like cells (MG 63) were exposed to the materials for 24 h through the use of medium conditioned by beta-tricalcium phosphate/bioactive glass. Cell viability was measured by MTT assay in the cells and the data obtained were submitted to ANOVA, Tukey's multiple comparison (p<0.05). The beta-TCP/7.5-BG promoted an increase of cell proliferation. The results suggest that compositions and processing method studied may provide appropriate materials for tissue engineering. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 10/00863-0 - Processing and characterization of B-TCP/Al2O3 nanostructured scaffolds for biomedical applications
Grantee:Eliandra de Sousa Trichês
Support type: Regular Research Grants
FAPESP's process: 11/09240-9 - Calcium phosphate cement otimization for application in orthopedics and tissue engineering
Grantee:Mariana Motisuke
Support type: Regular Research Grants
FAPESP's process: 11/17877-7 - Development of new polymeric scaffolds by electrospinning technique with incorporation of vertically aligned carbon nanotubes and nanohidroxyapatite for bone tissue regeneration
Grantee:Anderson de Oliveira Lobo
Support type: Research Grants - Young Investigators Grants