Functional dominance of different aged larvae of Bt-resistant Spodoptera frugiperda (Lepidoptera: Noctuidae) on transgenic maize expressing Vip3Aa20 protein

Autor(es)

Miraldo, Leonardo L. ; Bernardi, Oderlei ; Horikoshi, Renato J. ; Amaral, Fernando S. A. e ; Bernardi, Daniel ; Omoto, Celso

• Tipo de documento: Artigo Científico
• Fonte: CROP PROTECTION; v. 88, p. 65-71, OCT 2016.
• Citações Web of Science: 4

Resumo

Fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith), is the main target pest of transgenic maize expressing insecticidal proteins from Bacillus thuringiensis Berliner (Bt) in Brazil. To optimize resistance management strategies, we evaluated the functional dominance of different aged larvae of Bt-resistant FAW on Vip3Aa20 maize. We measured the survival and development of Vip3Aa20-resistant, -heterozygote, and -susceptible strains on MIR162 (expressing Vip3Aa20) and Bt11 x MIR162 x GA21 (expressing Vip3Aa20 and CrylAb) maize. The resistant strain, from neonate to sixth instar, showed more than 72% survival on Vip3Aa20 maize. From surviving larvae, more than 64 and 54% developed to pupae and adults, respectively. In contrast, heterozygote and susceptible strains showed no larval survival up to fourth instar, and less than 25% larval survival in the fifth and sixth instar on Vip3Aa20 maize. These larvae produced less than 21% of pupae and adults. The development time of FAW strains from neonate to-adult exposed to Vip3Aa20 maize was similar; however, the resistant strain showed an increase of 2 d when compared to those fed only non-Bt maize. In summary, the resistance of S. frugiperda to Vip3Aa20 maize is functionally recessive from neonate up to fourth instar larvae. However, high larval survival of resistant strain and some survival of heterozygote larvae in advanced instars on Vip3Aa20 maize were observed. These results will be important for designing insect resistance management to Bt maize plants expressing Vip3Aa20 protein in Brazil. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 11/22743-0 - Avaliação do risco de evolução da resistência de Spodoptera frugiperda (J. E. Smith) e Diatraea saccharalis (Fabricius) à proteína VIP3Aa20 expressa em milho geneticamente modificado no Brasil
• Beneficiário: Oderlei Bernardi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado