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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Nutrient availability shapes the microbial community structure in sugarcane bagasse compost-derived consortia

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Autor(es):
Mello, Bruno L. ; Alessi, Anna M. ; McQueen-Mason, Simon ; Bruce, Neil C. ; Polikarpov, Igor
Número total de Autores: 5
Tipo de documento: Artigo Científico
Fonte: SCIENTIFIC REPORTS; v. 6, DEC 12 2016.
Citações Web of Science: 15
Resumo

Microbial communities (MCs) create complex metabolic networks in natural habitats and respond to environmental changes by shifts in the community structure. Although members of MCs are often not amenable for cultivation in pure culture, it is possible to obtain a greater diversity of species in the laboratory setting when microorganisms are grown as mixed cultures. In order to mimic the environmental conditions, an appropriate growth medium must be applied. Here, we examined the hypothesis that a greater diversity of microorganisms can be sustained under nutrient-limited conditions. Using a 16 S rRNA amplicon metagenomic approach, we explored the structure of a compost-derived MC. During a five-week time course the MC grown in minimal medium with sugarcane bagasse (SCB) as a sole carbon source showed greater diversity and enrichment in lignocellulose-degrading microorganisms. In contrast, a MC grown in nutrient rich medium with addition of SCB had a lower microbial diversity and limited number of lignocellulolytic species. Our approach provides evidence that factors such as nutrient availability has a significant selective pressure on the biodiversity of microorganisms in MCs. Consequently, nutrient-limited medium may displace bacterial generalist species, leading to an enriched source for mining novel enzymes for biotechnology applications. (AU)

Processo FAPESP: 10/52362-5 - Targeted analysis of microbial lignocellulolytic secretomes: a new approach to enzyme discovery
Beneficiário:Igor Polikarpov
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 11/21608-1 - Identificação e caracterização de novas enzimas com potencial na digestão de biomassa lignocelulósica
Beneficiário:Bruno Luan Soares Paula de Mello
Linha de fomento: Bolsas no Brasil - Doutorado Direto