| Texto completo | |
| Autor(es): Mostrar menos - |
Hernandez-Montelongo, J.
;
Lucchesi, E. G.
;
Nascimento, V. F.
;
Franca, C. G.
;
Gonzalez, I.
;
Macedo, W. A. A.
;
Machado, D.
;
Lancellotti, M.
;
Moraes, A. M.
;
Beppu, M. M.
;
Cotta, M. A.
Número total de Autores: 11
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Materials Science & Engineering C-Materials for Biological Applications; v. 71, p. 718-724, FEB 1 2017. |
| Citações Web of Science: | 5 |
| Resumo | |
In recent years, a common strategy, to obtain more uniform and controlled synthesis of polyelectrolytes multi layers (PEMs), relies on a previous polyethylenimine (PEI) coating of the substrate surface. PEI is a synthetic cationic polymer which provides a positive charge distribution on the materials to be covered with PEMs. Despite being an important step, this pre-layer deposition is frequently overlooked and no comprehensive characterizations or deep discussions are reported in literature. In that sense, this work reports on the synthesis of a typical PEI film that works as a precursor for PEMs, and its detailed physicochemical characterization. As many PEMs are produced for antibacterial and biomedical applications, the cytotoxicity of the film was also tested using fibroblasts, and its antibacterial activity was studied using Staphylococcus aureus and Pseudomonas aeruginosa. Our results present the formation of an ultra-thin film of PEI with a thickness around 3.5 nm, and with a significant percent of NH3+ (35% of the total amount of N) in its chemical structure; NH3+ is a key chemical group because it is considered an important bacterial killer agent. The film was stable and did not present important cytotoxic effect for fibroblasts up to 7 days, contrary to other reports. Finally, the PEI film showed high antibacterial activity against the S. aureus strain: reductions in cell density were higher than 95% up to 24 h. (C) 2016 Elsevier B.V. All rights reserved. (AU) | |
| Processo FAPESP: | 13/02300-1 - Nanofios semicondutores: mecanismos de formação e aplicação em biossensores |
| Beneficiário: | Mônica Alonso Cotta |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 10/51748-7 - Análise estrutural e química de biofilmes de Xylella fastidiosa |
| Beneficiário: | Mônica Alonso Cotta |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |