Tissue Microarray Analysis Applied to Bone Diagenesis

Autor(es)

Mello, Rafael Barrios ; Regis Silva, Maria Regina ; Seixas Alves, Maria Teresa ; Evison, Martin Paul ; Guimaraes, Marco Aurelio ; Francisco, Rafaella Arrabaca ; Astolphi, Rafael Dias ; Miazato Iwamura, Edna Sadayo

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 7, JAN 4 2017.
• Citações Web of Science: 6

Resumo

Taphonomic processes affecting bone post mortem are important in forensic, archaeological and palaeontological investigations. In this study, the application of tissue microarray (TMA) analysis to a sample of femoral bone specimens from 20 exhumed individuals of known period of burial and age at death is described. TMA allows multiplexing of subsamples, permitting standardized comparative analysis of adjacent sections in 3-D and of representative cross-sections of a large number of specimens. Standard hematoxylin and eosin, periodic acid-Schiff and silver methenamine, and picrosirius red staining, and CD31 and CD34 immunohistochemistry were applied to TMA sections. Osteocyte and osteocyte lacuna counts, percent bone matrix loss, and fungal spheroid element counts could be measured and collagen fibre bundles observed in all specimens. Decalcification with 7% nitric acid proceeded more rapidly than with 0.5 M EDTA and may offer better preservation of histological and cellular structure. No endothelial cells could be detected using CD31 and CD34 immunohistochemistry. Correlation between osteocytes per lacuna and age at death may reflect reported age-related responses to microdamage. Methodological limitations and caveats, and results of the TMA analysis of post mortem diagenesis in bone are discussed, and implications for DNA survival and recovery considered. (AU)

• Processo FAPESP: 12/24918-4 - Caracterização histopatológica e metodologias efetivas para identificação humana por análise de DNA em ossadas exumadas
• Beneficiário: Rafael Dias Astolphi
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/12344-3 - "Análise de ossadas exumadas com perfil bioantropológico conhecido para estabelecimento dos melhores parâmetros antropológicos a serem aplicados à população brasileira em exames antropológicos forenses"
• Beneficiário: Raffaela Arrabaça Francisco
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/17901-0 - Investigação de preservação celular utilizando Tissue Microarray (TMA) na análise histopatológica de ossadas exumadas humanas
• Beneficiário: Rafael Barrios de Mello
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica